RUcore: Rutgers University Community Repository
Evolution of the circadian clock in the genus of neurospora
Descriptive
TitleInfo
Title
Evolution of the circadian clock in the genus of neurospora
SubTitle
conservation and divergence in the neurospora discreta species complex
Name
(type = personal)
NamePart
(type = family)
Wager
NamePart
(type = given)
Craig
NamePart
(type = date)
1986-
DisplayForm
Craig Wager
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Lee
NamePart
(type = given)
Kwangwon
DisplayForm
Kwangwon Lee
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Shain
NamePart
(type = given)
Daniel
DisplayForm
Daniel Shain
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Yakoby
NamePart
(type = given)
Nir
DisplayForm
Nir Yakoby
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Camden Graduate School
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2011
DateOther
(qualifier = exact);
(type = degree)
2011-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Subject
(authority = RUETD)
Topic
Biology
Subject
(authority = ETD-LCSH)
Topic
Circadian rhythms
Subject
(authority = ETD-LCSH)
Topic
Neurospora
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
Identifier
(type = local)
rucore10005600001
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Craig Wager
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
viii, 45 p. : ill.
Abstract
(type = abstract)
Circadian rhythms are daily oscillations in biological activities that have a periodicity of approximately 24 hours. Underlying these rhythms are multiple molecular
oscillators that interact to form the circadian clock of an organism. The filamentous fungi and eukaryotic model organism, Neurospora crassa, possesses a core circadian oscillator known as the FREQUENCY (FRQ)/WHITE-COLLAR (WC) oscillator (FWO). Recent studies have shown that homologous Neurospora clock genes are not universally conserved across the fungal kingdom. Here, we aimed to identify similarities
and differences at the sequence, molecular, and macroscopic level of the circadian clock in divergently related species to N. crassa. The Neurospora discreta species complex contains numerous reproductive species with isolates that have been collected from all
around the world, and extend the latitudinal boundaries of Neurospora by inhabiting regions as far North as Alaska, thus this clade provides an unique opportunity to study the evolution of the fungal clock to diverse local environments. Sequence comparison of the Neurospora discreta PS4b (8579) core clock homologs revealed a high degree of overall conservation with notable exceptions in the presence of additional WC complex consensus binding sites in frequency, suggesting possible differences in gene expression.
Rhythms in asexual development in Neurospora discreta sensu stricto are overtly circadian regulated and underlying this were oscillations in FRQ abundance and phosphorylation were robust. Rhythmic conidiation in N. discreta PS4b 8579 was observed in cycling and free-running environments, however this expression was reliant upon several environmental conditions in order to be visualized. Molecular analysis of FRQ appears to reveal rhythms in abundance with decreased amplitude. An Alaskan strain, N. discreta PS4b 9981, demonstrated blue light mediated photo-responses, but was arrhythmic under free-running conditions and temperature cycling conditions. Phenotypes in asexual development of N. discreta species range from robustly clock controlled to arrhythmic. Western analysis of FRQ in N. discreta sensu stricto and N. discreta PS4b 8579, suggests that these phenotypes might reflect differences in the FWO.
Based on data from the current study, we propose that these changes in clock regulation might have played a role for the N. discreta species to adapt to their local environments.
oscillators that interact to form the circadian clock of an organism. The filamentous fungi and eukaryotic model organism, Neurospora crassa, possesses a core circadian oscillator known as the FREQUENCY (FRQ)/WHITE-COLLAR (WC) oscillator (FWO). Recent studies have shown that homologous Neurospora clock genes are not universally conserved across the fungal kingdom. Here, we aimed to identify similarities
and differences at the sequence, molecular, and macroscopic level of the circadian clock in divergently related species to N. crassa. The Neurospora discreta species complex contains numerous reproductive species with isolates that have been collected from all
around the world, and extend the latitudinal boundaries of Neurospora by inhabiting regions as far North as Alaska, thus this clade provides an unique opportunity to study the evolution of the fungal clock to diverse local environments. Sequence comparison of the Neurospora discreta PS4b (8579) core clock homologs revealed a high degree of overall conservation with notable exceptions in the presence of additional WC complex consensus binding sites in frequency, suggesting possible differences in gene expression.
Rhythms in asexual development in Neurospora discreta sensu stricto are overtly circadian regulated and underlying this were oscillations in FRQ abundance and phosphorylation were robust. Rhythmic conidiation in N. discreta PS4b 8579 was observed in cycling and free-running environments, however this expression was reliant upon several environmental conditions in order to be visualized. Molecular analysis of FRQ appears to reveal rhythms in abundance with decreased amplitude. An Alaskan strain, N. discreta PS4b 9981, demonstrated blue light mediated photo-responses, but was arrhythmic under free-running conditions and temperature cycling conditions. Phenotypes in asexual development of N. discreta species range from robustly clock controlled to arrhythmic. Western analysis of FRQ in N. discreta sensu stricto and N. discreta PS4b 8579, suggests that these phenotypes might reflect differences in the FWO.
Based on data from the current study, we propose that these changes in clock regulation might have played a role for the N. discreta species to adapt to their local environments.
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10005600001.ETD.000061024
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3CJ8CVT
Genre
(authority = ExL-Esploro)
ETD graduate
Back to the top
Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Wager
GivenName
Craig
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-05-02 14:01:40
AssociatedEntity
Name
Craig Wager
Role
Copyright holder
Affiliation
Rutgers University. Camden Graduate School
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
Back to the top
Technical
FileSize
(UNIT = bytes)
6812672
OperatingSystem
(VERSION = 5.1)
windows xp
ContentModel
ETD
MimeType
(TYPE = file)
application/pdf
MimeType
(TYPE = container)
application/x-tar
FileSize
(UNIT = bytes)
6819840
Checksum
(METHOD = SHA1)
16dc3fb8d9d0ddc819b64ad4099eb4164428610d
Back to the top
Statistical Profile