Rutgers University Electronic Theses and Dissertations
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ETD
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Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Jennifer Czerniawski
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Form (authority = gmd)
electronic resource
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application/pdf
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text/xml
Extent
v, 126 p. : ill.
Abstract (type = abstract)
The dorsal and ventral subregions of the hippocampus are differentially involved in several of types of learning, including fear conditioning. For example, we have previously demonstrated that the integrity of ventral, but not dorsal, hippocampus is necessary for the acquisition and expression of trace fear conditioning while dorsal, but not ventral, hippocampus is critically involved in spatially-guided reinforced alternation (Czerniawski, Yoon & Otto, 2009). In contrast to the partially dissociable effects of either lesions or inactivation, however, several lines of research suggest that, in intact subjects, both subregions are normally involved in the acquisition of many hippocampal-dependent tasks. The present studies investigated the molecular basis of these forms of learning by determining whether NMDA receptor-mediated immediate early gene expression in dorsal vs. ventral hippocampus contributes to the acquisition and/or retention of trace and contextual fear conditioning. In the first set of experiments we examined the effect of NMDA receptor antagonism in dorsal or ventral hippocampus on the acquisition or expression of trace and contextual fear conditioning. Next we assessed if trace fear conditioning alters the transcription and/or translation of Arc, an immediate early gene thought to be critically involved in some forms of plasticity and learning. In addition we examined the effect of blocking Arc translation with antisense oligodeoxynucleotides on the acquisition of CS-US associations in this paradigm. Lastly, in the final experiment we determined if the learning induced increase in Arc translation is NMDA receptor-dependent. The results of these studies suggest that both NMDA-receptor antagonism and the infusion of antisense oligodeoxynucleotides for the immediate early gene Arc (activity-regulated cytoskeletal protein) into dorsal or ventral hippocampus impair the acquisition of contextual and trace fear conditioning. In addition, trace fear conditioning enhances both Arc transcription and translation. Finally, pre-training infusions of either Arc antisense oligodeoxynucleotides or the NMDA receptor antagonist APV block the learning-induced enhancement of Arc. Together these studies support the notion that NMDA-receptor mediated expression of the immediate early gene Arc in both dorsal and ventral hippocampus may underlie the acquisition of a variety of forms of hippocampal dependent learning.
Rutgers University. Graduate School - New Brunswick
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.