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Structure-function analysis of the non-helicase domain of Sgs1, the Bloom Syndrome ortholog from Saccharomyces cerevisiae

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Title
Structure-function analysis of the non-helicase domain of Sgs1, the Bloom Syndrome ortholog from Saccharomyces cerevisiae
Name (type = personal)
NamePart (type = family)
Tuberty-Vaughan
NamePart (type = given)
Lynda
NamePart (type = date)
1967-
DisplayForm
Lynda Tuberty-Vaughan
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Brill
NamePart (type = given)
Steven J
DisplayForm
Steven J Brill
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Vershon
NamePart (type = given)
Andrew
DisplayForm
Andrew Vershon
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
co-chair
Name (type = personal)
NamePart (type = family)
Mckim
NamePart (type = given)
Kim
DisplayForm
Kim Mckim
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
co-chair
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2011
DateOther (qualifier = exact); (type = degree)
2011-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Bloom’s Syndrome (BS) is a rare human disease characterized by genome instability and cancer predispostion. The gene mutated in BS, BLM, encodes a member of the RecQ family of DNA helicases. BLM is unique among this family because it is the only RecQ member conserved in unicellular eukaryotes. Apart from the DNA helicase domain, BLM/SGS1 orthologs contain a poorly characterized N-terminal domain of about 650 amino acids (aa). In yeast, this domain (Sgs1 1-652) is known to be physiologically important although it’s only known roles are to bind Top3 and Rmi1 through its N-terminal 100 aa, and in vitro single-strand DNA (ssDNA) binding, ssDNA annealing and strand exchange (SE) activities, through its SE domain (Sgs1 103-323) My research consisted of structure/function analyses of Sgs1 in its poorly characterized N-terminus of aa 323-652 and I found a domain that is important to Sgs1’s function. I interrogated this region by doing systematic deletions and tested these sgs1 mutants for complementation of synthetic lethality. The allele with the smallest deletion that did not complement sgs1slx4 synthetic lethality was found to be sgs1-∆386-621, and the allele with the smallest deletion that did not complement sgs1slx5, was found to be sgs1-∆500-621. I found that sgs1-∆386-621 is physiologically important also through the Top3 slow growth assay. Furthermore I believe that the difference in results between sgs1slx4 and sgs1slx5 will prove significant and warrants further investigation. I also used a BLM/GCN4 chimera where the SE domain was replaced with the coiled coil GCN4, and again tested alleles with systematic deletions within aa 323-652 in synthetic lethality assay. I found the smallest essential domain to be sgs1/gcn4-∆500-621 in the sgs1slx4 background, and interestingly the same deletion in sgs1-∆500-621 did grow weakly and complemented synthetic lethality. Therefore I postulate there is some interaction between the SE domain and aa 500-621 that could be physiologically important and again warrants further investigation.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_3301
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
ix, 76 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Lynda Tuberty-Vaughan
Subject (authority = ETD-LCSH)
Topic
Saccharomyces cerevisiae
Subject (authority = ETD-LCSH)
Topic
Genomics
Subject (authority = ETD-LCSH)
Topic
Oncogenic DNA viruses
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061532
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3TM79DH
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Tuberty-Vaughan
GivenName
Lynda
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2011-04-14 15:58:28
AssociatedEntity
Name
Lynda Tuberty-Vaughan
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
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Copyright protected
Availability
Status
Open
Reason
Permission or license
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