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Growth, genes, genomes
Descriptive
TitleInfo
Title
Growth, genes, genomes
SubTitle
iInsights into microbial respiration of arsenic and selenium
Name
(type = personal)
NamePart
(type = family)
Rauschenbach
NamePart
(type = given)
Ines
NamePart
(type = date)
1981-
DisplayForm
Ines Rauschenbach
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Häggblom
NamePart
(type = given)
Max M.
DisplayForm
Max M. Häggblom
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Kerkhof
NamePart
(type = given)
Lee
DisplayForm
Lee Kerkhof
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Bini
NamePart
(type = given)
Elisabetta
DisplayForm
Elisabetta Bini
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Yee
NamePart
(type = given)
Nathan
DisplayForm
Nathan Yee
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2011
DateOther
(qualifier = exact);
(type = degree)
2011-10
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Arsenic (As) and selenium (Se) are naturally occurring metalloids in the Earth’s crust. Their speciation is governed by the microbial communities in various environments which influences their mobility among the soil, water, and air interface. Microorganisms can utilize As and Se oxyanion as terminal electron acceptors in dissimilatory reduction. These organisms are ubiquitous and phylogenetically diverse. The objectives of the studies in this thesis were to gain an understanding of the metabolism of As and Se respiring bacteria, analyze the genes encoding enzymes involved in respiration and understand how these enzymes are regulated in the presence of various electron acceptors. We were able to isolate two novel As and Se respiring bacteria from different environments; from a wastewater treatment facility in Verona, NJ and an estuarine canal from Chennai, India. Based on 16S rRNA gene analysis, strain S4 was classified as a novel genus and species, Selenovibrio woodruffi and strain S5 as a novel species, Desulfurispirillum indicum. We analyzed the genome of D. indicum and examined the expression of putative reductases to further understand respiratory metabolism of As and Se oxyanions. Five molybdoenzyme genes were identified in the genome of strain S5, three of which we were annotated to encode for a respiratory arsenate reductase arr, periplasmic nitrate reductase nar, and respiratory nitrate reductase nap. Also, an arsenate resistance system, ars, was identified. We were not able to positively identify a selenate reductase gene. Gene expression studies revealed that arr was an inducible gene and the only gene highly expressed during arsenate respiration. Growth studies showed that selenate respiration was inhibited by nitrate. Lastly, we also enriched activated sludge samples for tellurium oxyanion respiring bacteria. Thus, we not only added novel, phylogenetically different organisms to the ever-increasing list of As and Se respiring microbes, we also provided insights into the genes and enzymes involved in As and Se respiration and how they are regulated.
Subject
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_3512
PhysicalDescription
Form
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electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xiii, 152 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Ines Rauschenbach
Subject
(authority = ETD-LCSH)
Topic
Selenium ores
Subject
(authority = ETD-LCSH)
Topic
Arsenic
Subject
(authority = ETD-LCSH)
Topic
Microbial respiration
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063577
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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NjNbRU
Identifier
(type = doi)
doi:10.7282/T3P849ZV
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Rauschenbach
GivenName
Ines
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-10-02 20:50:47
AssociatedEntity
Name
Ines Rauschenbach
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2011-10-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2013-10-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2013.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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30637056
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