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L1-guided differentiation of embryonic stem cells towards neural lineages
Descriptive
TitleInfo
Title
L1-guided differentiation of embryonic stem cells towards neural lineages
Name
(type = personal)
NamePart
(type = family)
Shih
NamePart
(type = given)
Hsuan Yu
NamePart
(type = date)
1986-
DisplayForm
Hsuan Yu Shih
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
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(type = family)
SHREIBER
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(type = given)
DAVID IRA
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DAVID IRA SHREIBER
Affiliation
Advisory Committee
Role
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(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Firestein
NamePart
(type = given)
Bonnie Lynne
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Bonnie Lynne Firestein
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Cai
NamePart
(type = given)
Li
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Li Cai
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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(authority = RULIB)
school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
(qualifier = exact)
2011
DateOther
(qualifier = exact);
(type = degree)
2011-10
CopyrightDate
(qualifier = exact)
2011
Place
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(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Embryonic stem cells are pluripotent cells that have the ability to differentiate into cell lineages from all three germ layers. However, the use of stem cells in therapeutics relies on the ability to control their differentiation. Studies have shown that implantation of undifferentiated ES cells into an injury site leads to their spontaneous differentiation and potential tumor formation. One method to control stem cell differentiation is through the design of biomaterials that mimic the natural microenvironment during development. Biomaterials can provide a microenvironment in which host as well as replacement therapeutic cells can reside. Controlling this microenvironment provides opportunities to present specific physical and soluble cues that control cell and tissue fate. Herein, we conjugate the cell adhesion molecule L1 to type I collagen to allow for its sustained, physiologically relevant presentation. L1 is a member of the immunoglobulin superfamily shared by neural and immune cells and has been shown to promote neurite extension as well as functional recovery in adult rats after contusion-induced spinal cord injury. In this study, we will investigate the role of L1 on mouse embryonic stem cells. We will assay the effects of L1 presentation on cell adhesion, proliferation, and most importantly differentiation of embryonic stem cells (mESCs). As L1 has a homophilic binding domain, we will study the effects of using a genetically modified mESCs that overexpress L1 in combination with our L1-grafted biomaterial. Collectively, these studies will provide greater insight into the role of designing materials to guide the differentiation of stem cells. These materials may be used as delivery mechanisms for stem cell therapeutics or scaffolds on which ones own stem cells can differentiate towards a particular required cell type or lineage.
Subject
(authority = RUETD)
Topic
Biomedical Engineering
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_3652
PhysicalDescription
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(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
ix, 28 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Hsuan Yu Shih
Subject
(authority = ETD-LCSH)
Topic
Embryonic stem cells—Research
Subject
(authority = ETD-LCSH)
Topic
Stem cells—Transplantation
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063652
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3X06641
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Shih
GivenName
Hsuan Yu
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-10-02 18:37:06
AssociatedEntity
Name
Hsuan Yu Shih
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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5825024
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5826560
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