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Growth behavior of Alicyclobacillus acidoterrestris as a function of strain and culture conditions

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TitleInfo
Title
Growth behavior of Alicyclobacillus acidoterrestris as a function of strain and culture conditions
Name (type = personal)
NamePart (type = family)
Merle
NamePart (type = given)
Jennifer M.
DisplayForm
Jennifer Merle
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Montville
NamePart (type = given)
Thomas J
DisplayForm
Thomas J Montville
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Carman
NamePart (type = given)
George
DisplayForm
George Carman
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Matthews
NamePart (type = given)
Karl R
DisplayForm
Karl R Matthews
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2012
DateOther (qualifier = exact); (type = degree)
2012-01
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Alicyclobacillus acidoterrestris is an acidophilic, spore-forming spoilage organism of concern for the fruit juice industry. The occurrence of bacterial spore-formers in low pH foods was thought to be insignificant. However, in recent years, spoilage of acidic juice by Alicyclobacillus was recognized and the seriousness of this situation began to be appreciated. A. acidoterrestris has been associated with commercially pasteurized fruit juices as well as other low pH, shelf-stable products such as bottled tea and isotonic drinks. It has been isolated from garden and forest soils and may be introduced into the manufacturing process through unwashed or poorly washed fruit. If spores are not destroyed by processing, they can germinate, grow, and spoil product. The purpose of this study was to develop an understanding of A. acidoterrestris growth as a function of strain, pH and temperature so that growth of A. acidoterrestris spores might be inhibited by environmental control and product formulation. Four strains of A. acidoterrestris were used to investigate the growth kinetics in response to pH (3.0, 4.0, 5.0) and temperatures (30, 37.5, 45ÂșC) by measuring the optical density (OD) every hour for 48 hours using a microtitre plate reader to develop the growth curves. The growth rates were calculated using the software program DMFit and were used to illustrate the relationship between the growth kinetics of A. acidoterrestris strains. When examining the generated data, we observed that there were some differences between growth kinetics of tested strains at various pH and temperature conditions. The differences were random and did not follow any specific trend. The use of a reference strain allows for greater availability and reproducibility. Accordingly, the ATCC 49025 strain is representative of the food isolates (strain N1100, N1102 and N1139) and can be used as a reference strain.
Subject (authority = RUETD)
Topic
Food Science
Subject (authority = ETD-LCSH)
Topic
Bacterial spores--Growth
Subject (authority = ETD-LCSH)
Topic
Fruit juices--Preservation
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_3745
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
x, 54 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Jennifer M. Merle
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064147
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3C828BT
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Merle
GivenName
Jennifer
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2012-01-02 13:58:38
AssociatedEntity
Name
Jennifer Merle
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

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1203200
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application/pdf
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application/x-tar
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Checksum (METHOD = SHA1)
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