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A comparison of corneal wound healing after UVB and nitrogen mustard exposure

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TitleInfo
Title
A comparison of corneal wound healing after UVB and nitrogen mustard exposure
Name (type = personal)
NamePart (type = family)
Po
NamePart (type = given)
Iris P.
NamePart (type = date)
1984-
DisplayForm
IRIS PO
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Laskin
NamePart (type = given)
Jeffrey
DisplayForm
Jeffrey Laskin
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Gordon
NamePart (type = given)
Marion
DisplayForm
Marion Gordon
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Gerecke
NamePart (type = given)
Donald
DisplayForm
Donald Gerecke
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Laskin
NamePart (type = given)
Debra
DisplayForm
Debra Laskin
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2012
DateOther (qualifier = exact); (type = degree)
2012-01
CopyrightDate (qualifier = exact)
2012
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Vesicants such as nitrogen mustard (NM) cause blistering of skin, similar to that caused by ultraviolet B (UVB) exposure from the sun. In the cornea, such injury occurs as microbullae at the basal surface of the basal epithelial cells, where the cells sit on their basement membrane. We hypothesize that the steps in healing are not identical after exposure to these two blistering agents, since UV injury heals well, but mustards often cause injuries that can induce chronic complications. Twenty five years after a mustard exposure recurrent erosions can still develop (Javadi et al., 2005). In an effort to understand the pathogenesis of mustard exposure we investigated whether a NM injury heals more slowly than an equivalent UVB injury. To accomplish this, corneal organ cultures were exposed to different levels of UVB to determine conditions that would produce a 24 hour post-UVB phenotype equivalent to the 24 hour post-exposure phenotype of a 60 minute exposure to 100 nmol NM. Corneal organ cultures were irradiated for various times to produce different UVB exposures: 5 minutes exposure resulted in 100 mJ/cm2; 20 minutes, 400 mJ/cm2; 40 minutes, 800 mJ/cm2; 60 minutes, 1200 mJ/cm2; 80 minutes, 1600 mJ/cm2; and 100 minutes, 2000 mJ/cm2. Corneas were embedded in O.C.T compound, frozen, and then sectioned for H&E staining. Micrographs of sections were overlapped to make composites covering the entire diameter of the cornea. From the composites, the degree of epithelial-stromal separation was calculated as a percentage of the entire width of the cornea. Our results demonstrated that, at 24 hour after a 60 minutes exposure to 100 nmol NM, the epithelial-stromal separation was 58 ± 13 % of the corneal diameter. Of the UVB exposures, a nearly equivalent phenotype, 60 ± 11 % separation between the cell layers, was produced by the 2000 mJ/cm2 UVB exposure. The time needed for healing (i.e., a return to epithelial-stromal structural integrity) was found to be 7 days for NM exposure. In contrast, the equivalently damaged UVB-exposed corneas recovered in only 5 days. Immunofluorescence analysis showed that the provisional matrix components SPARC, hevin, tenascin-C, thrombospondin-1, and fibrillin-1 persisted at least 1 day longer in the NM-exposed corneas than in UVB-exposed corneas.
Subject (authority = RUETD)
Topic
Toxicology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_3721
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xii, 95 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Iris P. Po
Subject (authority = ETD-LCSH)
Topic
Cornea--Diseases
Subject (authority = ETD-LCSH)
Topic
Cornea--Preservation
Subject (authority = ETD-LCSH)
Topic
Ultraviolet radiation
Subject (authority = ETD-LCSH)
Topic
Nitrogen mustards
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064162
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3KH0MBG
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
PO
GivenName
IRIS
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2011-12-06 23:29:33
AssociatedEntity
Name
IRIS PO
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2012-01-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2012-08-01
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after August 1st, 2012.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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4203008
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