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Diversity of biodegradative gene populations in aquatic sediments examined by gene-targeted metagenomics
Descriptive
TitleInfo
Title
Diversity of biodegradative gene populations in aquatic sediments examined by gene-targeted metagenomics
Name
(type = personal)
NamePart
(type = family)
Rodgers-Vieira
NamePart
(type = given)
Elyse Anne
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Elyse Rodgers-Vieira
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Zylstra
NamePart
(type = given)
Gerben
DisplayForm
Gerben Zylstra
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Young
NamePart
(type = given)
Lily
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Lily Young
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Vetriani
NamePart
(type = given)
Costantino
DisplayForm
Costantino Vetriani
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Kukor
NamePart
(type = given)
Jerome
DisplayForm
Jerome Kukor
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Launen
NamePart
(type = given)
Loren
DisplayForm
Loren Launen
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2012
DateOther
(qualifier = exact);
(type = degree)
2012-01
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Alkanes are common environmental pollutants in soil and water. The degradation of medium length n-alkanes is initiated under aerobic conditions by alkane monooxygenases which add one atom of molecular oxygen to the terminal carbon resulting in an alkanol product. Alkane monooxygenases fall into two distinct classes: the integral membrane bound AlkB family and the cytoplasmic cytochrome P450 family. Gene-targeted metagenomics was used to examine the microbial diversity and distribution of these two types of alkane monooxygenases in sediments in the United States and Central Asia. The Passaic River in Newark, New Jersey has a long history of industrial pollution making it an ideal site to study monooxygenase diversity. 16S rRNA and alkane monooxgyenase gene populations were analyzed by pyrosequencing to determine if sampling location on the river influenced the microbial community and if triplicate enrichments yield comparable results. Samples were collected at an arbitrary start point (0 meters) and at 10 and 1000 meters down the river. The replicates were similar to each other at two of the three sampling locations and differed slightly at 1000 meters. Sediments from rivers and streams in Central Asia were compared to determine if novel alkane monooxygenase families could be found in a largely unstudied geographic region. The 16S rRNA and monooxygenase gene communities recovered from sediment and enrichments originating from disparate environments with varied anthropogenic influence were compared by pyrosequencing. Novel alkane monooxygenase populations were recovered from sites in Central Asia and comparisons between sites showed that each population was distinct due to their distant geographic origins. The effect of salinity on alkane monooxygenase populations was examined in sediments obtained from Puerto Rico. Samples were collected from the Port of San Juan, an estuary, mangroves, and shore locations. Salinity was not the major determinant of alkane monooxygenase community composition in hexadecane enrichment cultures. The type of environment (mangrove compared to shore or port locations) had the greatest affect on the gene populations recovered.
Subject
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject
(authority = ETD-LCSH)
Topic
Alkanes
Subject
(authority = ETD-LCSH)
Topic
Microbial ecology
Subject
(authority = ETD-LCSH)
Topic
Biodegradation
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_3741
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xi, 118 p. : ill., maps
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Elyse Anne Rodgers-Vieira
Subject
(authority = ETD-LCSH)
Topic
Marine sediments
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064167
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T37943P3
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Rodgers-Vieira
GivenName
Elyse
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2011-12-20 17:33:50
AssociatedEntity
Name
Elyse Rodgers-Vieira
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
FileSize
(UNIT = bytes)
3406336
OperatingSystem
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windows xp
ContentModel
ETD
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application/pdf
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application/x-tar
FileSize
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3409920
Checksum
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