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Genome-wide screening and physiological responses of saccharomyces cerevisiae to the volatile organic compound 1-octen-3-ol
Descriptive
TitleInfo
Title
Genome-wide screening and physiological responses of saccharomyces cerevisiae to the volatile organic compound 1-octen-3-ol
Name
(type = personal)
NamePart
(type = family)
Pu
NamePart
(type = given)
David C.
NamePart
(type = date)
1984-
DisplayForm
David Pu
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Bennett
NamePart
(type = given)
Joan W.
DisplayForm
Joan W. Bennett
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Tumer
NamePart
(type = given)
Nilgun E.
DisplayForm
Nilgun E. Tumer
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
White, Jr.
NamePart
(type = given)
James F.
DisplayForm
James F. White, Jr.
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
White, Jr.
NamePart
(type = given)
James F.
DisplayForm
James F. White, Jr.
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2012
DateOther
(qualifier = exact);
(type = degree)
2012-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
A major fungal volatile organic compound (VOC) called 1-octen-3-ol or "mushroom alcohol," is produced by most fungal species and is responsible for much of their characteristic musty odor. We believe that fungal VOCs are serving as chemical compounds that signal within a single organism or signal within members of a single species. Also, fungal VOCs may cause “sick building syndrome” (SBS). In this study, we used Saccharomyces cerevisiae as a model for understanding 1-octen-3-ol toxicity on a genome-wide level. A yeast growth assay using solid media prepared in plates sealed with Parafilm was developed. Conditions were identified which permitted reproducible yeast growth measurements when cultures were exposed to 1-octen-3-ol, a challenge due to the volatile nature of the compound. At 48 hours, 300 ppm of 1-octen-3-ol was found to completely inhibit growth of BY4741, a wild type strain. To identify genes and pathways involved in 1-octen-3-ol resistance, we carried out a high-throughput viability assay using the non-essential yeast knockout (KO) library. Of the ~ 4976 strains, we found that 92 (1.8%) showed resistance to 300 ppm of 1-octen-3-ol of which 21 (22.8%) are related to endosome transport. More specifically, 1-octen-3-ol is very likely to inhibit cell grow by the ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway.
Subject
(authority = RUETD)
Topic
Plant Biology
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_3977
PhysicalDescription
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(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
vii, 38 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by David C. Pu
Subject
(authority = ETD-LCSH)
Topic
Volatile organic compounds--Toxicology
Subject
(authority = ETD-LCSH)
Topic
Saccharomyces cerevisiae
Subject
(authority = ETD-LCSH)
Topic
Yeast fungi--Growth
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065247
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3833QZ0
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Pu
GivenName
David
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-04-16 10:33:01
AssociatedEntity
Name
David Pu
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-05-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2014-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2014.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
FileSize
(UNIT = bytes)
2142720
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windows xp
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ETD
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application/pdf
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application/x-tar
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2150400
Checksum
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