Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_3987
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
x, 100 p. : ill.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = vita)
Includes vita
Note (type = statement of responsibility)
by Shadi Riazi
Abstract (type = abstract)
Bacterial vaginosis (BV) is one the most common infections in women of reproductive age which results from the replacement of healthy vaginal microflora mostly by anaerobic pathogens such as Gardnerella vaginalis, Prevotella bivia and Peptostreptococcus spp. Conventional treatment of BV with the antibiotics metronidazole and clindamycin, as recommended by the Centers for Disease Control and Prevention, does not eradicate all of the microorganisms associated with BV and many women remain colonized with G. vaginalis and other anaerobes and inhibit the healthy vaginal microflora such as Lactobacillus spp. Lactosporin, a natural antimicrobial protein produced by Bacillus coagulans ATCC 7050 have shown to be an effective and safe alternative for BV treatment. This dissertation describes the isolation, characterization, mode of action and safety of lactosporin. Lactosporin was found to be an anionic (pI=3.5-4), heat stable protein that its activity is highly pH dependent. Lactosporin inhibited G. vaginalis in well diffusion assay while it had no effect on healthy vaginal lactobacilli. Mode of action studies established that lactosporin inhibits G. vaginalis by forming pores in the cytoplasmic membrane, causing efflux of intracellular material and dissipation of the ΔpH component of the proton motive force (PMF). The saferty of lactosporin was evaluated in-vitro by using EpiVaginal[trade mark] ectocervical tissue model where it was shown to be a safe, non-toxic preparation for feminine care application.
Rutgers University. Graduate School - New Brunswick
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License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.