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A regulatory element for interneuron progenitors in the developing vertebrate central nervous system
Descriptive
TitleInfo
Title
A regulatory element for interneuron progenitors in the developing vertebrate central nervous system
Name
(type = personal)
NamePart
(type = family)
Tzatzalos
NamePart
(type = given)
Evangeline
NamePart
(type = date)
1983-
DisplayForm
Evangeline Tzatzalos
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Cai
NamePart
(type = given)
Li
DisplayForm
Li Cai
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Grumet
NamePart
(type = given)
Martin
DisplayForm
Martin Grumet
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
co-chair
Name
(type = personal)
NamePart
(type = family)
Firestein
NamePart
(type = given)
Bonnie
DisplayForm
Bonnie Firestein
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Zhou
NamePart
(type = given)
Renping
DisplayForm
Renping Zhou
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2012
DateOther
(qualifier = exact);
(type = degree)
2012-05
CopyrightDate
(qualifier = exact)
2012
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
The development of the central nervous system (CNS) is regulated by non-protein coding gene regulatory elements that control the expression of neural stem cell genes via the interaction of protein trans-acting factors. As a result of recent progress in neuroscience and biotechnology, valuable insight into neural cell growth has been attained from important components of the neural stem cell protein expression profile. However, the role of cis-regulatory elements (non-protein coding genomic DNA on the same molecule) in neural stem cells remains confounded. A cis-regulatory element of neural progenitors during vertebrate development has been identified and characterized. This regulatory element is a conserved, non-protein coding region located within the established neural stem cell gene, Notch1. Notch1 is expressed in radial glia, which are self-renewing, neural stem/progenitor cells with long processes that serve as scaffolds for neuronal migration. A conserved non-coding region in the Notch1 locus (i.e., Notch1CR2) is active exclusively in the ventral CNS during neurogenic periods. On a cellular level, it is active in asymmetrically dividing cells that give rise to GABAergic interneuron progenitors and interneurons. Notch1CR2 is a novel regulatory element for interneuron progenitors. In this thesis, four studies of Notch1CR2 are presented. In the first study, CNS-specific regulatory activity of Notch1CR2 is revealed during chick embryonic development using in ovo electroporation. Second, the temporal-spatial profile of Notch1CR2 activity is determined to be present in cells with an interneuron progenitor phenotype using a transgenic mouse model. Third, the molecular mechanism of Notch1CR2 is investigated, and potential binding trans-acting factors of Notch1CR2 are identified. Finally, Notch1CR2 reveals a change in the interneuron progenitor population in the reeler mutant mouse compared to the wildtype. Reeler is a mutant mouse with deficiencies in neuronal migration and lamination. The discovery and characterization of Notch1CR2 contributes to the current knowledge of gene regulatory elements involved in the neural stem cell decision-making process. Notch1CR2 has the potential to serve as a tool for studying interneurons in other neurodegenerative models or as a platform for engineering cells for transplantation in patients with interneuron deficiencies.
Subject
(authority = RUETD)
Topic
Biomedical Engineering
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_3858
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xvii, 132 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Evangeline Tzatzalos
Subject
(authority = ETD-LCSH)
Topic
Nerve tissue proteins
Subject
(authority = ETD-LCSH)
Topic
Nerve growth factor
Subject
(authority = ETD-LCSH)
Topic
Nervous system--Growth
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065283
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3GQ6WQ4
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Tzatzalos
GivenName
Evangeline
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-03-24 14:54:50
AssociatedEntity
Name
Evangeline Tzatzalos
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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