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Molecular and phenotypic characterization of Zn-resistant methylobacterium spp. in soil systems

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TitleInfo
Title
Molecular and phenotypic characterization of Zn-resistant methylobacterium spp. in soil systems
Name (type = personal)
NamePart (type = family)
Kist
NamePart (type = given)
Jennifer
NamePart (type = date)
1980-
DisplayForm
Jennifer Kist
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Tate, III
NamePart (type = given)
Robert L.
DisplayForm
Robert L. Tate, III
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Young
NamePart (type = given)
Lily Y.
DisplayForm
Lily Y. Young
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Yee
NamePart (type = given)
Nathan
DisplayForm
Nathan Yee
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Wawrik
NamePart (type = given)
Boris
DisplayForm
Boris Wawrik
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2012
DateOther (qualifier = exact); (type = degree)
2012-10
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Methylobacterium species, pink-pigmented facultatively methylotrophic bacteria (PPFMs), have the potential facilitate agricultural and bioremediation processes through plant growth promotion, zinc cycling and biofilm production. Phenotypic and genetic studies of Methylobacterium strains were conducted to increase our understanding of the roles and evolution of PPFMs and to develop methods for characterizing environmental isolates to help realize their potential. Chapter 1 describes the isolation of Methylobacterium isolates from a New Jersey agricultural soil. mxaF, the structural gene for the methanol dehydrogenase enzyme, was compared with the 16S rRNA gene during the characterization of 114 PPFM isolates from agricultural soil, forest soil and blueberry skin. PPFM isolate 16S rRNA sequences were too similar to distinguish them at the species level. 16S rRNA sequences clustered tightly phylogenetically whereas mxaF clustering paralleled phenotypic patterns. Soil type, pH and plant cover did not influence Methylobacterium geographic distribution or select for specific mxaF sequences, indicating a reason for the widespread nature and versatility of PPFMs in the environment. A phylogenetic survey of archived GenBank® sequences is presented in Chapter 2. mxaF, mxaF-like and mxaF’ sequences were included with xoxF and other pyrroloquinoline-quinone-(PQQ)-dependent methanol/ethanol dehydrogenase sequences. Methylobacterium mxaF sequences clustered tightly, even with other bacterial genera similar methylotrophy genes present. mxa gene family operon construction revealed evidence of horizontal gene transfer (HGT). HGT events could not be confirmed by examining G+C content of mxa genes. It is unclear whether the PPFM mxa operon construction pattern is the ancestor or the descendant of those of other genera. Biofilm production, a major mode of colonization for plant-associated bacteria, is addressed for Methylobacterium species in Chapter 3. The effects of various substrates and Zn concentrations on biofilm development were investigated. Low levels of Zn did not inhibit biofilm production but did affect time-dependently affect the amount of DNA per gram of biofilm material in a time-dependent manner. Chapter 4 addresses Zn tolerance in PPFMs. Isolates exbitied precipitation of a white solid, clearing zone production, or growth with neither phenomenon. Isolates solubilized hopeite, a Zn phosphate compound. This ability can potentially raise the bioavailability of Zn in soils and was not described for this genus prior to this research. Plant growth-promotion of red clover (Trifolium pratense) seeds and seedlings by PPFM isolates is described in Chapter 5. Not all Methylobacterium species promoted plant growth, indicating the importance of determining individual ability of PPFM isolates.
Subject (authority = RUETD)
Topic
Environmental Sciences
Subject (authority = ETD-LCSH)
Topic
Methylobacterium--New Jersey
Subject (authority = ETD-LCSH)
Topic
Growth (Plants)--Molecular aspects
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_4199
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xv, 228 p. : ill.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = vita)
Includes vita
Note (type = statement of responsibility)
by Jennifer Kist
Subject (authority = ETD-LCSH)
Topic
Growth (Plants)--New Jersey
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066844
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3M32TJK
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Kist
GivenName
Jennifer
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2012-08-24 01:32:51
AssociatedEntity
Name
Jennifer Kist
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2012-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2014-10-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2014.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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