This research aimed at developing zein-based ice nucleation films for the applications of frozen foods. Acetic acid was chosen as the solvent to cast zein films with flawless surface. Tributyl citrate (TBC) was mixed into zein films to various proportions and 10% TBC was most effective on improving mechanical properties resulting in 2.3-fold more flexibility and 5.8-fold more toughness compared to unplasticized zein films. But incorporation of 20% TBC significantly decreased the water absorption by 19% compared to 10% TBC. Thus, zein films with 20% TBC with good flexibility and water resistance was used in following experiments. The activity of ECINs was stable at pH between 4.0 and 9.0 and ionic strength between 0.01 M and 0.10 M. In the development of INFs, the optimum adsorption of ECINs on zein films surface occurred through layer-by-layer method at pH 7.0 and ionic strength of 0.05 M on UV/ozone-treated zein. A novel method was developed to quantify the activity of INFs, which revealed that the highest activity of zein-based INFs reached 175 units/mm2. The zein-based ice nucleation films (INFs) were used to wrap frozen bread dough during five freeze/thaw cycles. The high-activity INF was as effective as blending ECINs in improving the yeast survival by 40%, and consequently increased the specific volume of bread loaf by 25%. Furthermore, high-activity INFs prevented the dehydration of frozen dough and thus significantly reduced the crumb hardness by 36% and increased the crumb springiness by 1.25 times. On energy saving, zein-based INFs were less potential than blended ECINs. INFs only reduced the time of freezing by 6% compared to the 20% by blended ECINs. However, the total amount of ECINs used on wrapping a piece of dough was only about 1/200 of the amount through blending. In addition, ECINs immobilized on the zein films showed desirable stability to sustain at least fifteen repetitive uses on freezing water.
Subject (authority = RUETD)
Topic
Food Science
Subject (authority = ETD-LCSH)
Topic
Frozen foods--Packaging
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.