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Dynamics of gene expression profiling in liver following thermal injury and sepsis
Descriptive
TitleInfo
Title
Dynamics of gene expression profiling in liver following thermal injury and sepsis
Name
(type = personal)
NamePart
(type = family)
Yang
NamePart
(type = given)
Qian
NamePart
(type = date)
1981-
DisplayForm
QIAN YANG
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Androulakis
NamePart
(type = given)
Ioannis P.
DisplayForm
Ioannis P. Androulakis
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Berthiaume
NamePart
(type = given)
Francois
DisplayForm
Francois Berthiaume
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Ierapetritou
NamePart
(type = given)
Marianthi G
DisplayForm
Marianthi G Ierapetritou
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Cai
NamePart
(type = given)
Li
DisplayForm
Li Cai
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2012
DateOther
(qualifier = exact);
(type = degree)
2012-10
CopyrightDate
(qualifier = exact)
2012
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Burn injury leads to a prolonged inflammatory response in the body. Moreover, severe burn injuries are always associated with bacterial infections which cause more persistent inflammatory response, resulting in prolonged hyper-metabolism and hyper-catabolism on systemic level. Despite significant advances in patient care, morbidity and mortality remain high in those patients. The difficulty in developing new and more effective medications is due, in part, to our incomplete understanding of the underlying pathophysiology of the disease. Liver, the main organ regulating both the inflammation and metabolism plays a key role in responding to external injuries. Thus, analyzing the responses in liver to burn, infection and “double hits” injury from global perspective as well as in a timely manner may offer a molecular framework for study on the pathophysiology of systemic inflammation induced by injuries. The overall studies were divided into single injury in which the animals were subjected to single burn injury or single cecal ligation and puncture (CLP) injury individually and “double hits” injury in which the rats were subjected to a burn injury and subsequent CLP injury. Animals were sacrificed at various time points, and whole liver samples were analyzed using Affymetrix Rat Genome 230 2.0 Arrays. After identifying differentially expressed probesets in injury rats vs. sham over time, the concatenated data sets corresponding to these differentially expressed probesets in injury and sham were combined and analyzed using a “consensus clustering” approaches. Ingenuity Pathway Analysis (IPA) was used to functionally annotate genes, and RT-PCR was used to confirm microarray trends. Both single burn and CLP injury induces the activation of pro-inflammatory response, anti-inflammatory response, and enhanced synthesis of acute-phase proteins, increased metabolism and tissue damage. Genes which are directly in response to bacteria removal are only triggered in CLP injury. In double hits study, burn priming prior to CLP disrupts the transcriptional response in the liver to septic injury in the rat by altering the onset of anti-bacterial functions in the liver. In addition, burn enhanced hyper metabolic conditions through aggressive amino acid degradation at critical time points.
Subject
(authority = RUETD)
Topic
Chemical and Biochemical Engineering
Subject
(authority = ETD-LCSH)
Topic
Burns and scalds--Complications
Subject
(authority = ETD-LCSH)
Topic
Liver--Diseases
Subject
(authority = ETD-LCSH)
Topic
Protein microarrays
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_4129
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xii, 138 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Qian Yang
Subject
(authority = ETD-LCSH)
Topic
Gene expression
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067029
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3CJ8C7R
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
YANG
GivenName
QIAN
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-05-17 23:17:12
AssociatedEntity
Name
QIAN YANG
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-10-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2013-05-02
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 2nd, 2013.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
FileSize
(UNIT = bytes)
1627136
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windows xp
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ETD
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application/pdf
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application/x-tar
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1628160
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Statistical Profile