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Association of pluripotency gene promoter methylation with the chromosomal status of products of conception
Descriptive
TitleInfo
Title
Association of pluripotency gene promoter methylation with the chromosomal status of products of conception
Name
(type = personal)
NamePart
(type = family)
Lonczak
NamePart
(type = given)
Agnieszka
NamePart
(type = date)
1982-
DisplayForm
Agnieszka Lonczak
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Gao
NamePart
(type = given)
Nan
DisplayForm
Nan Gao
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Treff
NamePart
(type = given)
Nathan R.
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Nathan R. Treff
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
co-chair
Name
(type = personal)
NamePart
(type = family)
Rodriguez
NamePart
(type = given)
Alexis J.
DisplayForm
Alexis J. Rodriguez
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Dobrowolski
NamePart
(type = given)
Radek
DisplayForm
Radek Dobrowolski
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - Newark
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2013
DateOther
(qualifier = exact);
(type = degree)
2013-01
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Infertility affects one in six couples and often necessitates the use of assisted reproductive technology (ART). While ART is the most effective treatment, efficiency remains poor with less than 13% of transferred in vitro fertilization (IVF) derived embryos resulting in a live birth according to the Center for Disease Control. This has led to routine use of multiple embryo transfer to increase pregnancy rates. However, as a result of multiple embryo transfer, a significant proportion of IVF pregnancies involve multiples. Indeed, multiple gestation is the most common complication associated with ART and is now the primary focus of research and development in reproductive medicine. The ability to identify the embryo with true reproductive potential could overcome the need for multiple embryo transfer in order to achieve reasonable pregnancy rates from IVF. Differentiation and establishment of the trophectoderm lineage during preimplantation embryo development represents a potential target to identify new biomarkers of reproductive potential. Several gene promoters have already been shown to be differentially methylated in pluripotent versus differentiated cells. These promoters include: NANOG, PTPN6, RAB25, LYST, GBP3, MGMT, Oct4 and Elf5. The extent of methylation of these promoters was characterized after the development of a methodology for methylation sensitive restriction enzyme digestion followed by quantitative real-time PCR. Chromosomal aneuploidy is a well characterized marker of reproductive potential. The level of differentiation inferred from methylation status of these promoters was used to evaluate whether aneuploid and euploid conceptions possess unique levels of differentiation. Results indicate that GBP3 promoter methylation is significantly different in aneuploid relative to euploid conceptions supporting the concept that chromosomally normal embryos may differentiate more successfully than chromosomally abnormal embryos.
Subject
(authority = RUETD)
Topic
Biology
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_4442
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
vii, 24 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Agnieszka Lonczak
Subject
(authority = ETD-LCSH)
Topic
Human reproductive technology
Subject
(authority = ETD-LCSH)
Topic
Methylation
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10002600001.ETD.000067595
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - Newark Electronic Theses and Dissertations
Identifier
(type = local)
rucore10002600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3MS3RG4
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Lonczak
GivenName
Agnieszka
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-12-20 15:04:05
AssociatedEntity
Name
Agnieszka Lonczak
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - Newark
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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