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Development, characterization, and applications of self-assembling, photocrosslinkable collagen-based hydrogels
Descriptive
TitleInfo
Title
Development, characterization, and applications of self-assembling, photocrosslinkable collagen-based hydrogels
Name
(type = personal)
NamePart
(type = family)
Gaudet
NamePart
(type = given)
Ian Daniel
NamePart
(type = date)
1980-
DisplayForm
Ian Gaudet
Role
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(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Shreiber
NamePart
(type = given)
David
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David Shreiber
Affiliation
Advisory Committee
Role
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(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Firestein
NamePart
(type = given)
Bonnie
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Bonnie Firestein
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Moghe
NamePart
(type = given)
Prabhas
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Prabhas Moghe
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Burdick
NamePart
(type = given)
Jason
DisplayForm
Jason Burdick
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2013
DateOther
(qualifier = exact);
(type = degree)
2013-01
Place
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(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Abstract Development of functional soft-tissue engineered constructs for use in regenerative medicine is currently limited by homogeneity within scaffolds that fails to recapitulate the complex architecture that supports normal function in healthy tissues. Additionally, recent breakthroughs in our understanding the biomechanical cell-matrix interface have provided insight into the role of substrate compliance during development and in the pathophysiological environment. This thesis is the result of investigation into using type-I collagen as a base material for creating dynamic, self-assembling, mechanically and biochemically tunable 3D hydrogel scaffolds into which instructive cellular cues can be imparted anisotropically via the directed application of light. This overarching goal was approached by (1) evaluating extant methods for photonically manipulating type I collagen mechanical properties, which led us to the conclusion that published methods were inadequate for our purposes. Following this realization, we (2) developed a novel process for derivatizing free amines on collagen amino acid residues to reactive methacrylamide moieties, allowing robust spatiotemporal control of mechanical properties through photocrosslinking with long-wave UV light and the water-soluble photoinitiator Irgacure 2959. Thorough characterization of this material, collagen methacrylamide (CMA), provided the basis for multiple applications in the field of soft tissue engineering. Additionally, (3) CMA was used in conjunction with synthetic photopolymers in an effort to create a hybrid natural/synthetic hydrogel material. CMA was also (4) employed as a dynamic hydrogel scaffold which we showed could be used to culture a number of neurogenic stem and progenitor cell types with a focus on using photomodulation to impart instructive heterogeneity to the mechanical and biochemical microenvironment. Finally, (5) we used a computational modeling approach to explain interesting yet poorly understood material phenomena exhibited by CMA observed during characterization. Using sequence and structure based models of an optimized triple helical segment of type-I collagen, we obtained valuable insight into the role of amino acid electrostatic interactions in CMA thermodynamic behavior as well as in the context of understanding the biophysical mechanisms of native type I collagen self-assembly and stability.
Subject
(authority = RUETD)
Topic
Biomedical Engineering
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_4437
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xviii, 291 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Ian Daniel Gaudet
Subject
(authority = ETD-LCSH)
Topic
Colloids in medicine
Subject
(authority = ETD-LCSH)
Topic
Collagen
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067770
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3V123GG
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Gaudet
GivenName
Ian
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2012-12-19 13:43:07
AssociatedEntity
Name
Ian Gaudet
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ETD
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windows xp
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