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Mapping regulatory DNA that governs Drosophila eggshell patterning
Descriptive
TitleInfo
Title
Mapping regulatory DNA that governs Drosophila eggshell patterning
Name
(type = personal)
NamePart
(type = family)
Pope
NamePart
(type = given)
Nicole
NamePart
(type = date)
1986-
DisplayForm
Nicole Pope
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Yakoby
NamePart
(type = given)
Nir
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Nir Yakoby
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Kotchoni
NamePart
(type = given)
Simeon
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Simeon Kotchoni
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Lee
NamePart
(type = given)
Kwangwon
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Kwangwon Lee
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Camden Graduate School
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2013
DateOther
(qualifier = exact);
(type = degree)
2013-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Organogenesis relies on extensive tissue patterning by regulating the expression of genes in a spatiotemporal manner. During oogenesis, the follicle cells, a monolayer of epithelial cells surrounding the developing oocyte, are patterned to drive the formation of the Drosophila eggshell; an organ that shelters the developing embryo. While follicle cells’ patterning has been vastly documented, the regulatory domains that govern tissue patterning are mostly unknown. To find regulatory domains, we cross-listed 81 genes known to be expressed during oogenesis with the large collection of the Gerald M. Rubin (GMR) lines containing noncoding DNA fragments, and we found 19 common genes. These genes are represented by 223 GMR lines. Of great advantage, all GMR lines are driving the expression of a GAL4 thus providing an opportunity to screen these lines by crossing them to a GFP reporter gene. Of the tested GMR lines, 55 lines, (25%), express GFP during oogenesis, and 18 lines (33%), recapitulate the partial or full endogenous pattern of their corresponding genes. We found that regulatory information is enriched in certain positions of the genes’ locus. We also demonstrated the use of the new driver lines to disrupt morphologies of the eggshell and other tissues. Our comprehensive screen identified multiple regulatory DNA fragments that govern eggshell patterning.
Subject
(authority = RUETD)
Topic
Biology
Subject
(authority = ETD-LCSH)
Topic
Drosophila--Morphogenesis--Molecular aspects
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_4835
PhysicalDescription
Form
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electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note
Supplementary File: Expression patterns
Extent
vi, 42 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Nicole Pope
Subject
(authority = ETD-LCSH)
Topic
Genetic markers
Subject
(authority = ETD-LCSH)
Topic
DNA
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10005600001.ETD.000068608
RelatedItem
(type = host)
TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
Identifier
(type = local)
rucore10005600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T35Q4TQR
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Pope
GivenName
Nicole
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2013-05-09 13:24:02
AssociatedEntity
Name
Nicole Pope
Role
Copyright holder
Affiliation
Rutgers University. Camden Graduate School
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ETD
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windows xp
RULTechMD
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ETD
RULTechMD
(ID = TECHNICAL3)
ContentModel
ETD
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