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The sea urchin seawi and other argonaute family of proteins
Descriptive
TitleInfo
Title
The sea urchin seawi and other argonaute family of proteins
SubTitle
it’s a small RNA world after all
Name
(type = personal)
NamePart
(type = family)
Chuang
NamePart
(type = given)
Ling-shiang
NamePart
(type = date)
1976-
DisplayForm
Ling-shiang Chuang
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Bonder
NamePart
(type = given)
Edward M.
DisplayForm
Edward M. Bonder
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Rodriguez
NamePart
(type = given)
Alexis J.
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Alexis J. Rodriguez
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Friedman
NamePart
(type = given)
Wilma J.
DisplayForm
Wilma J. Friedman
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Maurel
NamePart
(type = given)
Patrice
DisplayForm
Patrice Maurel
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Hart
NamePart
(type = given)
Ronald P.
DisplayForm
Ronald P. Hart
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - Newark
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2013
DateOther
(qualifier = exact);
(type = degree)
2013-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
A fundamental question in studying embryonic development is to understand the regulation and integration of major developmental events, such as cell proliferation, cell specialization, cell interaction, and cell movement. Regulation of protein transcription, translation, and signaling during development can be modeled by the developmental gene regulatory network (GRN). In the sea urchin GRN, the animal-vegetal axis is established by polarized expression or localization of cell-fate determinants, such as beta-catenin nuclear localization in vegetal cells and bep4 expression in animal cells. Over the last decade, the discovery of small non-coding RNA as an effector of epigenetic, post-transcriptional, and translational regulation has led to reconsideration of the GRN. In this thesis, we investigated the role of argonaute/piwi and their association with small non-coding RNA in sea urchin development. We characterized the sea urchin argonaute/piwi proteins, examining their developmental expression profiles, characterizing the proteins present in seawi-associated MT-RNP complexes, and examining the small non-coding RNA populations in eggs, embryos, adult tissues, and MT-RNP complexes. We show seawi, sea urchin piwi, is expressed in germline lineage and stem cells in development, and seawi predominately associates with piRNA. Within the seawi-associated population of piRNAs, there are specific sets of piRNA sequences, which are complementary to the mRNA of cell-fate determinants bep4 and beta-catenin. These results lead to the potential for small non-coding RNA regulation of GRNs in sea urchin development. We also show that mi- and piRNAs are present in sea urchin sperm. This suggests that sperm has the ability to provide small non-coding RNAs to regulate embryonic developmental events. This finding challenges the current theory of sperm serving only as the delivery system for the paternal genome and provides new insight for a novel paternal regulation.
Subject
(authority = RUETD)
Topic
Biology
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_4859
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xi, 241 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Ling-shiang Chuang
Subject
(authority = ETD-LCSH)
Topic
Gene regulatory networks
Subject
(authority = ETD-LCSH)
Topic
Sea urchins--Embryos
Subject
(authority = ETD-LCSH)
Topic
RNA
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10002600001.ETD.000068665
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - Newark Electronic Theses and Dissertations
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rucore10002600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3BV7F70
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Chuang
GivenName
Ling-shiang
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2013-05-13 17:42:43
AssociatedEntity
Name
Ling-shiang Chuang
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - Newark
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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