Effects of De Novo protein synthesis on cell spreading, formation of cell-cell contacts, and co-localization of proteins at cell contacts in IAR-2 cells
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Effects of De Novo protein synthesis on cell spreading, formation of cell-cell contacts, and co-localization of proteins at cell contacts in IAR-2 cells
Rat epithelial cells were treated with translational inhibitors in order to study their effect on cell spreading, the cell’s ability to form lasting cell-cell contacts, and protein co-localization at cell contacts. When cells were treated with translation inhibitors they were found to have similar cell lengths as wild-type, untreated cells up to the 3 hour time point. After 3 hours the translational-inhibitor-treated cells were found to decrease in size while the untreated cells continued to grow. The co-localization of E-cadherin and actin were studied at cell contacts between two cells and it was found in inhibitor-treated cells the co-localization of the two proteins actually increased compared to wild-type cells. Lastly, a 30-fold increase in the ability to make lasting cell contacts was found in wild-type, untreated cells compared to inhibitor treated cells. Altogether these data demonstrate that de novo protein synthesis is needed in order for cells to continue to properly grow and spread, for proteins to be synthesized and co-localize properly at cell-cell contacts, and for cells to make lasting, non-random, cell contacts.
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Biology
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Cells--Growth--Regulation
Subject (authority = ETD-LCSH)
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Cell interaction
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