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Evaluation of assays for epoxides in oxidized lipids

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TitleInfo
Title
Evaluation of assays for epoxides in oxidized lipids
Name (type = personal)
NamePart (type = family)
Liao
NamePart (type = given)
Chen-Hsiang
NamePart (type = date)
1985-
DisplayForm
Chen-Hsiang Liao
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Schaich
NamePart (type = given)
Karen M
DisplayForm
Karen M Schaich
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
HO
NamePart (type = given)
CHI-TANG
DisplayForm
CHI-TANG HO
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Hartman
NamePart (type = given)
Thomas G
DisplayForm
Thomas G Hartman
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2013
DateOther (qualifier = exact); (type = degree)
2013-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Epoxides have long been recognized as lipid oxidation products, and there is recent evidence that epoxides may be as or more important than hydroperoxides under many conditions. Nevertheless, epoxides are seldom analyzed when monitoring oxidative degradation, at least in part because there are few established analytical procedures. The goal of this thesis research, therefore, was to evaluate four available epoxide assays for accuracy, sensitivity, stoichiometry, reproducibility, and handling requirements, and from the results provide analytical protocols and practical guidelines for selection and application of assays for lipid epoxides. AOCS standard HBr titration of epoxides, nitrobenzylpyridine (NBP) reaction with colorimetric endpoint, diethyl dithiocarbamate (DETC) complexation with high pressure liquid chromatography (HPLC) separation and quantitation of adducts, and 1H nuclear magnetic resonance (NMR) analysis of epoxides were evaluated using epoxybutane, epoxyhexene, and epoxydecene as standards. The HBr assay is too insensitive (detects 0.0075-0.1M) for following lipid epoxides in foods and biological materials. In addition, the reaction must be run under inert atmosphere to prevent non-specific oxidation of the Br, and HBr degrades so rapidly that frequent restandardization is necessary. Nitrobenzylpyridine assay is more sensitive, detecting 0.5 mM epoxides. However, the NBP reaction response varied considerably with time and temperature of reaction and with epoxide structure (increased with epoxide chain length). Hence, a different standard must be used for each epoxide analyzed, and selection of an appropriate standard for epoxides of unknown structure is problematic. For the DETC assay, reaction response was linear from 1M to 1 mM for all three epoxide standards (R2 > 0.99) and oxidized methyl linoleate (R2 > 0.94), and increased with epoxide chain length. HPLC analysis of adducts allows differentiation and quantitation of individual epoxides, so can provide important information about oxidation chemistry as well as quantitation. NMR offers the advantage of direct analysis of oils and extracts, detects micromolar epoxides, and clearly distinguishes epoxides from other oxidation products in lipids. Response curves were linear with concentration of epoxide standards, oxidized corn oil, and oxidized methyl linoleate (R2> 0.98). The DETC-HPLC and NMR assays hold the greatest promise for routine analysis of epoxides in oxidized lipids.
Subject (authority = RUETD)
Topic
Food Science
Subject (authority = ETD-LCSH)
Topic
Epoxy compounds--Research
Subject (authority = ETD-LCSH)
Topic
Oxidation
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_4699
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note
Supplementary File:
Extent
xiii, 122 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = vita)
Includes vita
Note (type = statement of responsibility)
by Chen-Hsiang Liao
Subject (authority = ETD-LCSH)
Topic
Lipids--Research
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000068905
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3DV1HG9
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Liao
GivenName
Chen-Hsiang
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2013-04-15 07:41:39
AssociatedEntity
Name
Chen-Hsiang Liao
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2013-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2015-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2015.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
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windows xp
RULTechMD (ID = TECHNICAL2)
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ETD
RULTechMD (ID = TECHNICAL3)
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ETD
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