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Escherichia coli O157:H7 cell surface moieties trigger plant defense response influencing survival of that pathogen on plants

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TitleInfo
Title
Escherichia coli O157:H7 cell surface moieties trigger plant defense response influencing survival of that pathogen on plants
Name (type = personal)
NamePart (type = family)
Seo
NamePart (type = given)
Seung-Wook
NamePart (type = date)
1977-
DisplayForm
SEUNG-WOOK SEO
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Matthews
NamePart (type = given)
Karl R.
DisplayForm
Karl R. Matthews
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Chikindas
NamePart (type = given)
Michael L.
DisplayForm
Michael L. Chikindas
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Schaffner
NamePart (type = given)
Donald W.
DisplayForm
Donald W. Schaffner
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Annous
NamePart (type = given)
Bassam A.
DisplayForm
Bassam A. Annous
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2013
DateOther (qualifier = exact); (type = degree)
2013-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Previous studies have shown that human pathogens are able to colonize plants epiphytically and endophytically by utilizing similar mechanisms as phytopathogens. In addition, it has been suggested that colonization of plants by human pathogens induces plant defense responses, although some of their pathogenic mechanisms differ from those of phytopathogens. However, knowledge about the mechanism by which human pathogens colonize and persist on or in fruits and vegetables is limited compared to the research in plant pathogens. Also it is not well understood what factors are involved in intimate association of foodborne pathogens with plants and the plant response to enteric bacteria. To address these questions, we investigated whether Escherichia coli O157:H7 cell surface structures induce plant host defense responses and subsequently affect colonization or survival of the pathogen on plants. Also, the influence of growth medium or exposure conditions on bacterial cell surface structures, particularly in exopolysaccharides, was investigated, and subsequent impacts on plant defense responses and bacterial survival on plants were also examined. Among the pathogen associated molecular patterns (PAMPs), flagella made a substantial impact on survival of E. coli O157:H7 on the Arabidopsis plant. Curli were recognized by the Arabidopsis plant and induced plant defense responses. E. coli O157:H7 mutants lacking flagella or curli induced lower PR (pathogenesis-related) genes based on the weak β-glucuronidase (GUS) activity compared to flagella or curli positive E. coli O157:H7, resulting in increased survivals of those mutants on the plant. E. coli O157:H7 grown in Luria-Bertani (LB) broth supplemented with manure extracts showed a significant 58% increase in capsular polysaccharides (CPS) production as compared to cells grown in LB medium alone. Exposure of E. coli cells to soil and manure extracts also stimulated the CPS compared to the non-exposed control. Plants inoculated with E. coli O157:H7 with a greater amounts of CPS resulted in less inductions of PR genes compared to those with less CPS, contributed to the enhanced survivals on the Arabidopsis plant. Cell surface structures of E. coli O157:H7 have a significant impact on the induction of differential plant defense responses, thereby impacting survival of the pathogen on plants. Growth medium or exposure environment conditions showed a great impact on bacterial CPS, which enabled the human pathogen to persist longer on the plant by possibly evading plant defense responses.
Subject (authority = RUETD)
Topic
Food Science
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_4526
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
vii, 92 p. : ill.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = vita)
Includes vita
Note (type = statement of responsibility)
by Seung-Wook Seo
Subject (authority = ETD-LCSH)
Topic
Escherichia coli O157:H7
Subject (authority = ETD-LCSH)
Topic
Plants--Disease and pest resistance--Genetic aspects
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000068965
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T30000NM
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
SEO
GivenName
SEUNG-WOOK
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2013-02-19 12:58:03
AssociatedEntity
Name
SEUNG-WOOK SEO
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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