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The antibacterial mode of action and properties of Ib-AMP1, a plant-derived antimicrobial peptide, against Escherichia coli O157:H7
Descriptive
TitleInfo
Title
The antibacterial mode of action and properties of Ib-AMP1, a plant-derived antimicrobial peptide, against Escherichia coli O157:H7
Name
(type = personal)
NamePart
(type = family)
Wu
NamePart
(type = given)
Wen-Hsuan
NamePart
(type = date)
1983-
DisplayForm
Wen-Hsuan Wu
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
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(type = family)
Matthews
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(type = given)
Karl R
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Karl R Matthews
Affiliation
Advisory Committee
Role
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(authority = RULIB)
chair
Name
(type = personal)
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(type = family)
Di
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(type = given)
Rong
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Rong Di
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Advisory Committee
Role
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(authority = RULIB)
co-chair
Name
(type = personal)
NamePart
(type = family)
Chikindas
NamePart
(type = given)
Mikhael L
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Mikhael L Chikindas
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Renye
NamePart
(type = given)
John A
DisplayForm
John A Renye
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2013
DateOther
(qualifier = exact);
(type = degree)
2013-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
The continual occurrence of foodborne outbreaks along with the consumer demand for use of fewer traditional antimicrobial agents in foods has driven research interests in development of plant-derived antimicrobial agents (pAMPs) for use in food and food processing. Ib-AMP1 is a pAMP isolated from seeds of Impatiens balsamina. Previous studies indicated that it is a broad spectrum pAMP and the therapeutic index against eight human pathogens was 23.5; however, for future utilization, other antibacterial properties and mode of action must be elucidated. The purpose of this dissertation was to investigate the antibacterial properties and mode of action of Ib-AMP1 against Escherichia coli O157:H7, a foodborne pathogen that has been continually associated with foodborne outbreaks. The study design provided insight on the implantation and potential application of Ib-AMP1; a specific docking site or ligand-receptor relationship was not studied. The results demonstrated that Ib-AMP1 exhibited bactericidal activity against E. coli O157:H7, Salmonella enterica serovar Newport, Pseudomonas aeruginosa and Staphylococcus aureus. Ib-AMP1 at lethal concentrations (1X and 2X MIC) resulted in 1.46 to 2.69 log reduction of viable cells and prevented outgrowth when tested against low (103 CFU/mL) and medium (106 CFU/mL) E. coli O157:H7 populations. Ib-AMP1 at 2X MIC failed to inhibit and prevent outgrowth when cell numbers were 109 CFU/mL. No residual activity of Ib-AMP1 was apparent following interaction of the peptide with bacteria or the medium. Ib-AMP1 concentration less than 100 µg/mL showed little or no inhibition of human cell proliferation including human small intestine, colon and liver cells, which are associated with oral consumption of an AMP. The mode of action study demonstrated that a concentration dependent effect of Ib-AMP1 on the E. coli O157:H7 cell membrane occurred. Ib-AMP1 treatments resulted in efflux of K+ and ATP, suggesting pores of sufficient size to allow efflux of large molecules. The efflux of intracellular components may be associated with damage to the outer membrane and dissipation of cytoplasmic membrane potential. Results of this study suggest Ib-AMP1 is bactericidal interfering within outer and inner membrane integrity permitting efflux of ATP and interfering with intracellular biosynthesis of DNA, RNA, and protein.
Subject
(authority = RUETD)
Topic
Food Science
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_4574
PhysicalDescription
Form
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electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xiv, 158 p. : ill.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Wen-Hsuan Wu
Subject
(authority = ETD-LCSH)
Topic
Antibacterial agents
Subject
(authority = ETD-LCSH)
Topic
Food industry and trade
Subject
(authority = ETD-LCSH)
Topic
Escherichia coli O157:H7
Subject
(authority = ETD-LCSH)
Topic
Food--Microbiology
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000069007
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3RR1WTD
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Wu
GivenName
Wen-Hsuan
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2013-04-02 21:08:12
AssociatedEntity
Name
Wen-Hsuan Wu
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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