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Building plus strand viral RNA replication platforms
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Hsu, Nai-Yun. Building plus strand viral RNA replication platforms. Retrieved from https://doi.org/doi:10.7282/T3B27S8N

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TitleBuilding plus strand viral RNA replication platforms
NameHsu, Nai-Yun (author); Altan-Bonnet, Nihal (chair); Friedman, Wilma (internal member); Gao, Nan (internal member); Nagy, Peter (outside member); Rutgers University; Graduate School - Newark
Date Created2013
Other Date2013-10 (degree)
SubjectBiology, Phosphoinositides, RNA viruses, RNA--Synthesis, Viruses--Reproduction
Extentxiii, 166 p. : ill.
DescriptionMany RNA viruses remodel intracellular membranes of their hosts to generate specialized sites for RNA replication. How membranes are remodeled and what properties make them conducive for replication are unknown. Here we show how RNA viruses can manipulate multiple components of the cellular secretory pathway to generate organelles specialized for replication that are distinct in protein and lipid composition from the host cell. Specific viral proteins modulate effector recruitment by Arf1 GTPase and its guanine nucleotide exchange factor GBF1, promoting preferential recruitment of phosphatidylinositol-4-kinase IIIβ (PI4KIIIβ) to membranes over coat proteins, yielding uncoated phosphatidylinositol-4-phosphate (PI4P) lipid-enriched organelles. The PI4P-rich lipid microenvironment is essential for both enteroviral and flaviviral RNA replication; PI4KIIIβ inhibition interferes with this process. The potential mechanism by which PI4P mediated viral RNA replication are either tethering RdRP protein to replication membranes or regulating the RdRP and its precursor protein enzymatic activity. Our findings reveal how RNA viruses can selectively exploit specific elements of the host to form specialized organelles where cellular phosphoinositide lipids are key to regulating viral RNA replication.
NotePh.D.
NoteIncludes bibliographical references
NoteIncludes vita
Noteby Nai-Yun Hsu
Genretheses, ETD doctoral
Persistent URLhttps://doi.org/doi:10.7282/T3B27S8N
Languageeng
CollectionGraduate School - Newark Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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