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Development of a novel system for the expression on intracellular proteins
Descriptive
TitleInfo
Title
Development of a novel system for the expression on intracellular proteins
Name
(type = personal)
NamePart
(type = family)
Basant
NamePart
(type = given)
Ankita
NamePart
(type = date)
1985-
DisplayForm
ANKITA BASANT
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Marcotrigiano
NamePart
(type = given)
Joseph
DisplayForm
Joseph Marcotrigiano
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Stock
NamePart
(type = given)
Ann
DisplayForm
Ann Stock
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Arnold
NamePart
(type = given)
Eddy
DisplayForm
Eddy Arnold
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2013
DateOther
(qualifier = exact);
(type = degree)
2013-10
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Mammalian cell expression has become an important recombinant protein production system for a wide variety of applications because of the cells capacity for proper posttranslational modification and molecular structure assembly. In the past, mammalian expression has been optimized for secreted, glycosylated proteins. However, intracellular protein expression leaves yet another layer of complexity to be solved when expressing proteins in mammalian cells. There is a set of challenges that associates with intracellular protein expression due to the post-translational modifications involved with the bioactivity of the protein. The benefits to cytoplasmic protein production in mammalian cells, particularly for eukaryotic proteins, are very significant. Improvements in this area have been slow, however, due to limited development of the cell culture processes needed for low-cost and higher-throughput expression in mammalian cells. Here, we describe the development of a novel robust protein expression system using recombination cloning, suspension cells and stable lentiviral transfection to decrease the time and lower cost that can be combined to make mammalian cells much more amenable for routine recombinant protein expression.
Subject
(authority = RUETD)
Topic
Chemistry and Chemical Biology
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_5015
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
viii, 67 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Ankita Basant
Subject
(authority = ETD-LCSH)
Topic
Proteins--Chemical modification
Subject
(authority = ETD-LCSH)
Topic
Proteins
Subject
(authority = ETD-LCSH)
Topic
Mammals--Cytology--Research
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3736NXJ
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
BASANT
GivenName
ANKITA
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2013-09-19 10:52:33
AssociatedEntity
Name
ANKITA BASANT
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2013-10-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2015-10-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2015.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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Statistical Profile