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The role of glutamine in the function and cytokine secretion of 3T3-L1 adipocytes and RAW264.7 macrophages

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TitleInfo
Title
The role of glutamine in the function and cytokine secretion of 3T3-L1 adipocytes and RAW264.7 macrophages
Name (type = personal)
NamePart (type = family)
Dori
NamePart (type = given)
Samantha Ann
NamePart (type = date)
1984-
DisplayForm
Samantha Dori
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Watford
NamePart (type = given)
Malcolm
DisplayForm
Malcolm Watford
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Shapses
NamePart (type = given)
Sue A.
DisplayForm
Sue A. Shapses
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Storch
NamePart (type = given)
Judith
DisplayForm
Judith Storch
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Henderson
NamePart (type = given)
Gregory C.
DisplayForm
Gregory C. Henderson
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2013
DateOther (qualifier = exact); (type = degree)
2013-10
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Obesity is associated with many medical disorders including type 2 diabetes and certain cancers. During obesity there is increased infiltration of classically activated (M1) macrophages into adipose tissue which is associated with the development of insulin resistance and eventually type 2 diabetes. Macrophages require glutamine to survive but have limited expression of glutamine synthetase (GS), the only enzyme capable of producing glutamine de novo, thus glutamine must be obtained from external sources. During obesity blood flow is limited in adipose tissue hence bringing to question where M1 macrophages obtain their glutamine. Mature adipocytes express high amounts of GS and are known to release glutamine. Therefore glutamine from adipocytes may contribute to the infiltration of M1 macrophages into adipose tissue during obesity. We found that murine RAW264.7 macrophages express low levels of GS and have an absolute requirement for large amounts of exogenous glutamine for proliferation and secretion of proinflammatory cytokines MCP-1 (monocyte chemoattractant protein-1) and MIP-1α (macrophage inflammatory protein-1α). To determine if adipocyte-derived glutamine could support macrophage viability and function, mature 3T3-L1 murine adipocytes and RAW264.7 cells were cocultured for up to 48h in media without added glutamine and with a chemical inhibitor of GS. RAW264.7 cells survived and proliferated in the absence of exogenous glutamine when cocultured with mature 3T3-L1 adipocytes but did not survive in the absence of added glutamine when cocultured with 3T3-L1 preadipocytes or with mature 3T3-L1 adipocytes where GS was inhibited. In addition, when mature 3T3-L1 cells were cocultured with RAW264.7 cells in the absence of glutamine and with the GS inhibitor, the secretion of RANTES (regulated on activation normal T cell expressed and secreted) and Eotaxin-1 were negatively affected while secretion of MCP-1 was slightly affected. The lower secretion of RANTES, Eotaxin-1 and MCP-1 may have been due to RAW264.7 cells in cocultures dying in the absence of glutamine. These findings support the notion that glutamine produced by adipocytes may contribute to the viability and cytokine secretion of adipose tissue macrophages during obesity which may lead to the development of insulin resistance and type 2 diabetes.
Subject (authority = RUETD)
Topic
Nutritional Sciences
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5030
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xiii, 102 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Samantha Ann Dori
Subject (authority = ETD-LCSH)
Topic
Macrophages--Activation
Subject (authority = ETD-LCSH)
Topic
Glutamine synthetase
Subject (authority = ETD-LCSH)
Topic
Glutamine
Subject (authority = ETD-LCSH)
Topic
Obesity
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3XG9P6P
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Dori
GivenName
Samantha
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2013-09-23 15:59:59
AssociatedEntity
Name
Samantha Dori
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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