When food is transported at ambient temperatures for extended periods of time, or when power is lost during natural disasters; foodborne pathogens can multiply. Current US Food and Drug Administration (FDA) Model Food Code guidelines state that food can be kept out of temperature control for up to 4 h or up to 6 h if the food product starts at an initial 41°F (5°C) and the temperature does not exceed 70°F (21°C). This project validates existing ComBase computer models for Salmonella spp. growth under changing temperature conditions in raw ground beef as model system, using scenarios that would exceed Food Code guidelines. This thesis is separated into a literature review describing FDA Model Food Code guidelines, Salmonella prevalence and concentration in ground beef, and dynamic models for bacterial growth (I) and experimental validation of ComBase computer models for Salmonella spp. growth in raw ground beef (II). The growth rate of a 5-strain cocktail of Salmonella spp. meat isolates was inoculated in 20% fat ground beef at a concentration of 4-log CFU/g. Inoculated ground beef samples were temperature abused for different lengths of time and to different maximum temperatures. The temperature profiles represent loss of proper refrigeration, warming and then cooling following a linear temperature gradient. A total of 9 different conditions were studied. Results show that when maximum temperatures were low, there was generally good agreement between the ComBase models and experiments. When maximum temperatures were closer to the optimum growth temperature for Salmonella (37°C), predictive models were fail-safe. It appears that faster cooling times limit the growth of Salmonella, so rapidly cooling foods (e.g. in a freezer) after extended temperature abuse can work as a risk mitigation measure. Validation of these models will be useful to extension professionals advising consumers, restaurateurs transporting food in unrefrigerated vehicles, and retailers facing a power outage. These finding may also be useful to those seeking to improve the science base of the FDA Model Food Code.
Subject (authority = RUETD)
Topic
Microbial Biology
Subject (authority = ETD-LCSH)
Topic
Beef--Quality
Subject (authority = ETD-LCSH)
Topic
Salmonella
Subject (authority = ETD-LCSH)
Topic
Temperature control
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5070
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
vi, 40 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Jennifer A. McConnell
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
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