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Recombination hotspot activity in Drosophila melanogaster

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TitleInfo
Title
Recombination hotspot activity in Drosophila melanogaster
Name (type = personal)
NamePart (type = family)
Mehdi
NamePart (type = given)
Mahrose
NamePart (type = date)
1980-
DisplayForm
Mahrose Mehdi
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Padgett
NamePart (type = given)
Richard W
DisplayForm
Richard W Padgett
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
McKim
NamePart (type = given)
Kim S
DisplayForm
Kim S McKim
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Singson
NamePart (type = given)
Andrew
DisplayForm
Andrew Singson
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2013
DateOther (qualifier = exact); (type = degree)
2013-10
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Sexual reproduction depends on the success of faithful chromosome transmission during meiosis to yield viable gametes. Crucial to proper meiosis is the process of recombination between paternal and maternal chromosomes which ensures normal homologous chromosome segregation. Errors in number and location of the recombination events are known to be one of the leading causes of nondisjunction and aneuploidy. Recombination events tend to cluster in certain regions of the genome where the frequency of recombination is high compared to the average recombination rate. These regions are called recombination hotspots. The identification of these hotspots will bring us closer to understanding the etiology of nondisjunction. We used transposon insertion at defined sites to test for recombination hotspots in Drosophila melanogaster. The first method employs two transposable element bearing fly strains that have the insertion at sites flanking the proposed hotspot. The number of recombination events in the interval between the two transposon insertion sites is used to detect hotspots. The second strategy is to generate a double strand break (DSB) by mobilizing the excision of the transposon from a precise location. By monitoring the outcomes of DSB repair event at these loci, sites that have a higher recombination rates can be detected. Unfortunately, both methods did not reveal hotspot activity. Studies in yeast and mammals have uncovered a few genes that have a large effect on the distribution and pattern of recombination events. In Saccharomyces cerevisiae, Set1 complex (also known as COMPASS complex) has been shown to decreases DSB frequencies at > 80% of DSB sites genome-wide and cause changes in their localization. We want to know if Set1 complex plays a major role in controlling DSB sites in Drosophila Melanogaster also. RNAi mediated knockdowns of subunits of the Set1 complex are used to investigate if these flies show a reduction of DSBs. Although Set1 complex knockdowns did not have a significant effect on the DSBs, they may play a role in the repair of DSBs and oocyte development.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5120
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
iv, 72 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Mahrose Mehdi
Subject (authority = ETD-LCSH)
Topic
Genetic recombination--Research
Subject (authority = ETD-LCSH)
Topic
Drosophila melanogaster--Reproduction
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T33J3B0T
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Mehdi
GivenName
Mahrose
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2013-10-01 16:47:13
AssociatedEntity
Name
Mahrose Mehdi
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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