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Evaluating stucturally different pectic oligosaccharides in inhibiting adhesion of e. coli 0157:H7 to human gut epithelial cells in vitro
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Vakkalanka, Malathi Srilakshmi. Evaluating stucturally different pectic oligosaccharides in inhibiting adhesion of e. coli 0157:H7 to human gut epithelial cells in vitro. Retrieved from https://doi.org/doi:10.7282/T3ST7MW8

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TitleEvaluating stucturally different pectic oligosaccharides in inhibiting adhesion of e. coli 0157:H7 to human gut epithelial cells in vitro
NameVakkalanka, Malathi Srilakshmi (author); Yam, Kit L (chair); HOTCHKISS, ARLAND T (co-chair); LIU, LINSHU (co-chair); Rutgers University; Graduate School - New Brunswick
Date Created2013
Other Date2013-10 (degree)
SubjectFood Science, Bacteria--Adhesion, Escherichia coli O157:H7, Oligosaccharides, Epithelial cells
Extentvii, 37 p. : ill.
DescriptionBacterial adhesion to glycosylated cellular surfaces is a major concern in human health and disease. Inhibition of bacterial adhesion by suitable carbohydrates may lead to an anti-adhesion therapy as a novel prophylactic approach against bacterial infections and a potential alternative to the use of antibiotics. Selections of six pectic oligosaccharides derived from citrus peel albedo, which were different in terms of their monosaccharide composition and physical properties, were evaluated for their ability to interfere with the adhesion of Escherichia coli O157:H7 to HT29 cells in vitro. Attachment was determined in the human HT29 cell line by viable count of adherent bacteria. Most of the pectic oligosaccharides in buffer at pH 7.2 were anti-adhesive at a dose of 0.001 - 0.05 mg/ml, reducing adhesion of E.coli by 50 - 90% and concentrations of 0.5 - 5 mg/ml resulted in less than 50% reduction of adhesion to no effect. Based on the results, lower concentrations were more effective in reducing adhesion when compared to the higher concentrations. The pectic oligosaccharides with a homogalacturonan structure, low molecular weight and lower degree of esterification were the most effective in reducing the adhesion when compared to the oligosaccharides with an arabinose rich rhamnogalacturonan structure with higher molecular weight and higher degree of esterification. These results show that the pectic oligosaccharides with different monosaccharide composition and physical properties can display a wide range of anti-adhesive activity.
NoteM.S.
NoteIncludes bibliographical references
Noteby Malathi Srilakshmi Vakkalanka
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T3ST7MW8
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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