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Evaluating stucturally different pectic oligosaccharides in inhibiting adhesion of e. coli 0157:H7 to human gut epithelial cells in vitro

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TitleInfo
Title
Evaluating stucturally different pectic oligosaccharides in inhibiting adhesion of e. coli 0157:H7 to human gut epithelial cells in vitro
TitleInfo (type = alternative)
Title
Evaluating structurally different pectic oligosaccharides in inhibiting adhesion of e. coli 0157:H7 to human gut epithelial cells in vitro
Name (type = personal)
NamePart (type = family)
Vakkalanka
NamePart (type = given)
Malathi Srilakshmi
NamePart (type = date)
1987-
DisplayForm
Malathi Sr Vakkalanka
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Yam
NamePart (type = given)
Kit L
DisplayForm
Kit L Yam
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
HOTCHKISS
NamePart (type = given)
ARLAND T
DisplayForm
ARLAND T HOTCHKISS
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
co-chair
Name (type = personal)
NamePart (type = family)
LIU
NamePart (type = given)
LINSHU
DisplayForm
LINSHU LIU
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
co-chair
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2013
DateOther (qualifier = exact); (type = degree)
2013-10
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Bacterial adhesion to glycosylated cellular surfaces is a major concern in human health and disease. Inhibition of bacterial adhesion by suitable carbohydrates may lead to an anti-adhesion therapy as a novel prophylactic approach against bacterial infections and a potential alternative to the use of antibiotics. Selections of six pectic oligosaccharides derived from citrus peel albedo, which were different in terms of their monosaccharide composition and physical properties, were evaluated for their ability to interfere with the adhesion of Escherichia coli O157:H7 to HT29 cells in vitro. Attachment was determined in the human HT29 cell line by viable count of adherent bacteria. Most of the pectic oligosaccharides in buffer at pH 7.2 were anti-adhesive at a dose of 0.001 - 0.05 mg/ml, reducing adhesion of E.coli by 50 - 90% and concentrations of 0.5 - 5 mg/ml resulted in less than 50% reduction of adhesion to no effect. Based on the results, lower concentrations were more effective in reducing adhesion when compared to the higher concentrations. The pectic oligosaccharides with a homogalacturonan structure, low molecular weight and lower degree of esterification were the most effective in reducing the adhesion when compared to the oligosaccharides with an arabinose rich rhamnogalacturonan structure with higher molecular weight and higher degree of esterification. These results show that the pectic oligosaccharides with different monosaccharide composition and physical properties can display a wide range of anti-adhesive activity.
Subject (authority = RUETD)
Topic
Food Science
Subject (authority = ETD-LCSH)
Topic
Bacteria--Adhesion
Subject (authority = ETD-LCSH)
Topic
Escherichia coli O157:H7
Subject (authority = ETD-LCSH)
Topic
Oligosaccharides
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5085
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
vii, 37 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Malathi Srilakshmi Vakkalanka
Subject (authority = ETD-LCSH)
Topic
Epithelial cells
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3ST7MW8
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Vakkalanka
GivenName
Malathi Sr
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2013-10-03 18:07:35
AssociatedEntity
Name
Malathi Sr Vakkalanka
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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