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A functional analysis of liver‐ and intestinal fatty acid binding proteins in the intestinal enterocyte

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TitleInfo
Title
A functional analysis of liver‐ and intestinal fatty acid binding proteins in the intestinal enterocyte
SubTitle
studies in null mice
Name (type = personal)
NamePart (type = family)
Gajda
NamePart (type = given)
Angela Marie
DisplayForm
Angela Gajda
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Storch
NamePart (type = given)
Judith
DisplayForm
Judith Storch
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Brasaemle
NamePart (type = given)
Dawn L
DisplayForm
Dawn L Brasaemle
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Henderson
NamePart (type = given)
Gregory C
DisplayForm
Gregory C Henderson
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Quadro
NamePart (type = given)
Loredana
DisplayForm
Loredana Quadro
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Blaner
NamePart (type = given)
William S
DisplayForm
William S Blaner
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2014
DateOther (qualifier = exact); (type = degree)
2014-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
The fatty acid binding protein (FABP) family consists of 14-15 kD cytoplasmic proteins, which are abundantly expressed in various mammalian tissues. In the enterocyte, two FABPs are present: Liver-type FABP (LFABP; FABP1), and Intestinal FABP (IFABP; FABP2). Previous studies in chow-fed mice did not reveal marked differences between IFABP or LFABP null mice, thus we hypothesized that feeding high-fat diets would provide a lipid overload to the intestine, perhaps revealing phenotypic differences that would provide insight into the individual functions of LFABP and IFABP. We directly compared IFABP−/−, LFABP−/−, and WT mice fed low or high fat diets. Changes in mucosal lipid metabolism were observed, with a decrease in FA incorporation into triacylglycerol (TG) relative to phospholipid (PL) in IFABP−/− mice, and reduced monoacylglycerol incorporation in TG relative to PL and reduced FA oxidation in LFABP−/− mice. Striking changes in whole body phenotypes were found: LFABP−/− mice fed a high-fat diet had greater weight gain and fat mass relative to WT, while IFABP−/− mice remained lean. Respiratory exchange ratios suggest that high-fat fed LFABP−/− mice preferentially oxidize lipids, while IFABP−/− mice preferentially metabolize carbohydrate as a fuel source. IFABP-/- mice are healthy with a lean phenotype, whereas LFABP-/- mice are obese but are otherwise metabolically normal. To further elucidate the individual and overlapping functions of enterocyte FABPs, we generated a novel strain of mice that were null for both IFABP and LFABP (DKO). The phenotype of DKO mice fed a high-fat diet was integrated between LFABP-/- and IFABP-/- mice, with body weights and composition in between the single knockout mice. DKO mice fed a high-fat diet were similar to IFABP-/- mice in primarily using carbohydrate for energy. It was also observed that DKO mice were less active relative to WT mice. Importantly, there were no differences between groups in fecal lipids, suggesting that LFABP and IFABP are not involved with bulk FA uptake into the intestine. Overall, the results of these studies strongly suggest that IFABP and LFABP have different functions in intestinal lipid metabolism, which result in downstream alterations in whole body energy metabolism.
Subject (authority = RUETD)
Topic
Nutritional Sciences
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5438
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xv, 179 p. : ill.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Angela Marie Gajda
Subject (authority = ETD-LCSH)
Topic
Fatty acid-binding proteins
Subject (authority = ETD-LCSH)
Topic
Liver--Metabolism--Endocrine aspects
Subject (authority = ETD-LCSH)
Topic
Mice as laboratory animals
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3P26WFV
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Gajda
GivenName
Angela
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-04-10 17:12:46
AssociatedEntity
Name
Angela Gajda
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2015-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2015.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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ETD
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windows xp
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