Staff View
Biotransformation of aniline, para-chloroaniline and pentachloronitrobenzene and environmental implications

Descriptive

TitleInfo
Title
Biotransformation of aniline, para-chloroaniline and pentachloronitrobenzene and environmental implications
Name (type = personal)
NamePart (type = family)
Li
NamePart (type = given)
Yun
NamePart (type = date)
1982-
DisplayForm
Yun Li
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Fennell
NamePart (type = given)
Donna E
DisplayForm
Donna E Fennell
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Huang
NamePart (type = given)
Weilin
DisplayForm
Weilin Huang
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Häggblom
NamePart (type = given)
Max M
DisplayForm
Max M Häggblom
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Mack
NamePart (type = given)
Elizabeth Erin
DisplayForm
Elizabeth Erin Mack
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2014
DateOther (qualifier = exact); (type = degree)
2014-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
This dissertation describes research on biotransformation of aniline and para-chloroaniline (PCA) under anoxic and anaerobic conditions in sediments and groundwater aquifer solids recovered from a chemical industrial site. Four locations were examined: lightly and highly contaminated freshwater canal sediments, and lightly and highly contaminated aquifer sediments. Aquifer and canal microcosms showed loss of aniline under nitrate-, Fe (III)- and sulfate-amended conditons, and under methanogenic conditions. Expected intermediates of aniline biotransformation were not detected. Only 5 to 10 percent of 14C was recovered as CO2 from 14C-labelled aniline during a mineralization test. Thus, definitive pathways of biodegradation were not determined for aniline. PCA loss was observed in nitrate-amended microcosms. Reductive dechlorination of PCA was observed in canal microcosms under methanogenic conditions and was concurrent with stoichiometric aniline production. Dechlorination was slow, and was only observed after increasing the PCA concentration to 1500 µM and adding the electron donor/hydrogen source, lactate. PCA loss was not observed under methanogenic conditions in aquifer sediments. Combined with the observation of aniline loss in methanogenic microcosms, these results support the possibility for complete dechlorination of monochloroaniline to aniline and subsequent degradation of aniline in canal sediments. The bacterial phylotypes present in nitrate-aniline-amended microcosms clustered with known aniline degraders (Delftia) and nitrate-reducers (Acidovorax and Variovorax). The dominant phylotype in the most active aniline-amended transfers had high similarity to Magnetospirillum, a genus that is capable of nitrate reduction and degradation of aromatic pollutants. The phylotypes identified in PCA-dechlorinating microcosms included Dehalobacter, a dechlorinator that has been linked to dechlorination of chlorinated benzenes at the same site. This work highlights the potential for anaerobic transformation of aniline and PCA at this industrial site and is one of only a few studies that have identified bacterial community members in sediments and enrichments that carry out these transformations. More work is needed to conclusively identify the biological pathways responsible for the loss of aniline. Finally, a mixed culture containing Dehalococcoides mccartyi strain 195 was tested for ability to transform pentachloronitrobenzene to chlorinated anilines. The culture did not dechlorinate PCA. PCNB was abiotically transformed to pentachloroaniline, which was reductively dechlorinated to dichloroaniline.
Subject (authority = RUETD)
Topic
Environmental Sciences
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5489
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xl, 355 p. : ill.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Yun Li
Subject (authority = ETD-LCSH)
Topic
Aniline--Biodegradation
Subject (authority = ETD-LCSH)
Topic
Chloroaniline--Biodegradation
Subject (authority = ETD-LCSH)
Topic
Quintozene--Biodegradation
Subject (authority = ETD-LCSH)
Topic
Biotransformation (Metabolism)
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3TH8K0S
Genre (authority = ExL-Esploro)
ETD doctoral
Back to the top

Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Li
GivenName
Yun
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-04-14 09:21:55
AssociatedEntity
Name
Yun Li
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2016-05-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 30th, 2016.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
Back to the top

Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
Back to the top
Version 8.3.13
Rutgers University Libraries - Copyright ©2021