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Identification of oxidative products of pterostilbene and 3'-hydroxypterostilbene in vitro and evaluation of anti-inflammatory and anti-cancer cell proliferative activity
Descriptive
TitleInfo
Title
Identification of oxidative products of pterostilbene and 3'-hydroxypterostilbene in vitro and evaluation of anti-inflammatory and anti-cancer cell proliferative activity
Name
(type = personal)
NamePart
(type = family)
Liu
NamePart
(type = given)
Boya
NamePart
(type = date)
1988-
DisplayForm
BOYA LIU
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
HO
NamePart
(type = given)
CHI-TANG
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CHI-TANG HO
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
HUANG
NamePart
(type = given)
QINGRONG
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QINGRONG HUANG
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Hartman
NamePart
(type = given)
Thomas G.
DisplayForm
Thomas G. Hartman
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2014
DateOther
(qualifier = exact);
(type = degree)
2014-05
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Pterostilbene (trans-3,5-dimethoxy-4ʹ-hydroxystilbene) is an important phenolic compound which is reported as a potential anti-oxidative, anti-inflammatory, and anti-cancer agent. It is a natural compound and belongs to the group of phytoalexins, agents produced by plants to fight infections like chemicals and fungal. In nature, pterostilbene can be oxidized by peroxidase from fungus, and peroxidase also exists in human body. So it may undergo oxidative process in human as well. 3ʹ-hydroxypterostilbene is another natural phenolic compound and also is a metabolite of pterostilbene identified in mouse. The two adjacent hydroxyl groups in its molecule indicate that it may have a stronger bioactivity than pterostilbene. To understand the possible oxidative reaction and major products in metabolic process, this research use peroxidase and phosphate-citrate buffer to simulate the peroxidation of pterostilbene and 3ʹ-hydroxypterostilbene in vitro. The major oxidative products were identified as pterostilbene trans dehydrodimer and 3ʹ-hydroxypterostilbene trans dehydrodimer by LC/MS and NMR. The anti-inflammatory and anti-cancer cell proliferative activity of three compounds, pterostilbene, 3ʹ-hydroxypterostilbene, and pterostilbene trans dehydrodimer, was evaluated in this research. In anti-inflammatory evaluation, Griess assay was used to measure the nitric oxide production in RAW264.7 cells which were treated with the three compounds at different concentration. In anti-cancer cell proliferative study, MTT assay was used to measure the cell viability of two cancer cell lines: drug-sensitive HL-60 leukemia cell line and drug-resistant MDA-MB231 breast cancer cell line. According to our data, 3ʹ-hydroxypterostilbene had a significant anti-inflammatory and anti-cancer cell proliferative activity.
Subject
(authority = RUETD)
Topic
Food Science
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_5515
PhysicalDescription
Form
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electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xi, 61 p. : ill.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Boya Liu
Subject
(authority = ETD-LCSH)
Topic
Phenols in the body
Subject
(authority = ETD-LCSH)
Topic
Cancer--Chemoprevention
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
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(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3PR7T9H
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
LIU
GivenName
BOYA
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2014-04-15 05:14:38
AssociatedEntity
Name
BOYA LIU
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2014-05-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2016-05-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 30th, 2016.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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