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Actions of the sulfur mustard analogue, nitrogen mustard, on mouse skin and post exposure treatment with Connexin 43 antisense oligodeoxynucleotides

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TitleInfo
Title
Actions of the sulfur mustard analogue, nitrogen mustard, on mouse skin and post exposure treatment with Connexin 43 antisense oligodeoxynucleotides
Name (type = personal)
NamePart (type = family)
Wang
NamePart (type = given)
James Daniel
NamePart (type = date)
1986-
DisplayForm
James Wang
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Gerecke
NamePart (type = given)
Donald R
DisplayForm
Donald R Gerecke
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Chang
NamePart (type = given)
Yokechen
DisplayForm
Yokechen Chang
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Gordon
NamePart (type = given)
Marion K
DisplayForm
Marion K Gordon
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Witz
NamePart (type = given)
Gisela
DisplayForm
Gisela Witz
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2014
DateOther (qualifier = exact); (type = degree)
2014-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Nitrogen mustard (NM), a less potent vesicant analog of sulfur mustard (SM), has very similar biological activity and function compared with SM. Connexin 43 (Cx43), the ubiquitously expressed gap junction protein in the skin, plays a significant regulatory role in wound repairs. In non-diabetic rodents and humans, there is a downregulation of Cx43 and an up-regulation of Connexin 26 and 30 (Cx26 and Cx30) in keratinocytes at the wound edges. The SKH-1 hairless mouse is a widely used wound healing model which has been used in SM research. The studies described here employed this model to examine the hypothesis that the changes in expression of Cx43 which are involved in diabetic and incisional/excisional wound healing play a role in the healing of NM chemical burn injuries as well. In addition, we hypothesize that Cx43 antisense oligodeoxynucleotides (Cx43 asODN) will facilitate the healing process after NM exposure as has been observed in the treatment of diabetic and incisional/excisional wounds. Using the SKH-1 mouse dorsal skin model, the present studies demonstrated that Cx43 was downregulated by NM at days 1, 3, 7, and 10 post-NM exposure, in a manner similar to that normally occurring in excisional/incisional wounds. In addition, Cx43 was further downregulated significantly by Cx43 asODN at day 3 and day 7. In iii contrast, Cx26 and Cx30 were upregulated by NM for days 1, 3, 7, and 10 just has been observed in the diabetic and incisional/excisional wound models. In addition, Cx43 asODN significantly reduced expression of Cx26 and Cx30. Furthermore, Cx43 asODN reduced levels of the proinflammatory mediator interleukin 1B (IL-1B), COX-2 and increased interleukin 10 (IL-10), an anti-inflammatory cytokine. Finally, the histological examination of tissue sections of the mouse skin demonstrated that Cx43 asODN reduced inflammatory cell infiltration, hyperplasia, and thickening of the stratum corneum. The data suggest that Cx43, Cx26, and Cx30 play important roles in the healing of NM injuries in the SKH-1 hairless mouse dorsal skin model similar to that of the incisional/excisional wound model. In addition, post exposure treatment with Cx43 asODN leads to reduce expression of Cx43, Cx26, and Cx30 and facilitate wound repair.
Subject (authority = RUETD)
Topic
Toxicology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5487
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xiii, 165 p. : ill.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by James Daniel Wang
Subject (authority = ETD-LCSH)
Topic
Toxicology
Subject (authority = ETD-LCSH)
Topic
Nitrogen mustards
Subject (authority = ETD-LCSH)
Topic
Wounds and injuries--Treatment
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T30K26VD
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Wang
GivenName
James
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-04-14 09:33:13
AssociatedEntity
Name
James Wang
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2015-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2015.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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