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Comparative genomics of the stem transcriptome from grain and sweet sorghum
Descriptive
TitleInfo
Title
Comparative genomics of the stem transcriptome from grain and sweet sorghum
Name
(type = personal)
NamePart
(type = family)
Calviño Torterolo
NamePart
(type = given)
Martĺn
DisplayForm
Martĺn Calviño Torterolo
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Messing
NamePart
(type = given)
Joachim
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Joachim Messing
Affiliation
Advisory Committee
Role
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chair
Name
(type = personal)
NamePart
(type = family)
Maliga
NamePart
(type = given)
Pal
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Pal Maliga
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Tumer
NamePart
(type = given)
Nilgun
DisplayForm
Nilgun Tumer
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Chen
NamePart
(type = given)
Kevin
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Kevin Chen
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
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2014
DateOther
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(type = degree)
2014-10
CopyrightDate
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2014
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
The current dissertation relates to comparative genomics of grain and sweet sorghum, in particular, to their stem’s transcriptome at the time of flowering, when soluble sugars accumulate more abundantly in the sweet sorghum cultivar Rio than in the grain sorghum cultivar BTx623. The accumulation of soluble sugars in the stem of sorghum is a valuable agronomic trait because their fermentation into ethanol is currently being used as source of biofuel. High soluble sugar content in stems is a trait also present in the closely related grass sugarcane. Thus, it is reasonable to assume that sweet sorghum and sugarcane may use the same gene products that leads to high soluble sugar content is stems. My dissertation consists of five chapters, the results of which are five publications as first author. In Chapter 1 I summarized the current status of sweet sorghum genomics and highlighted future research directions. My scientific contribution to the field was also mentioned. In Chapters 2 and 3 I described the first characterization of the stem’s transcriptome from grain and sweet sorghum cultivars using sugarcane Affymetrix arrays, and the use of this transcriptome data to develop molecular markers based on the differences in hybridization intensity from grain and sweet sorghum RNAs to the arrays. In Chapter 4, I described the first characterization of the small RNA component of the stem from grain sorghum BTx623 and sweet sorghum Rio cultivars, and from F2 plants derived from their cross that segregated for sugar content and flowering time. I was able to identify the microRNA family miR169, whose expression co-segregated with sugar content in stems. I also discovered nine new microRNAs in the sorghum genome. In Chapter 5 I described the genomic comparison of MIR169 gene clusters among five different grasses and identified five new MIR169 gene copies in the sorghum genome.
Subject
(authority = RUETD)
Topic
Plant Biology
Subject
(authority = ETD-LCSH)
Topic
Grain--Genome mapping
Subject
(authority = ETD-LCSH)
Topic
Sorghum--Genome mapping
Subject
(authority = ETD-LCSH)
Topic
Botanical chemistry
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_5978
PhysicalDescription
Form
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electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xvi, 248 p. : ill.)
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Martĺn Calviño Torterolo
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3K35S35
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Calviño Torterolo
GivenName
Martĺn
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2014-09-30 22:31:07
AssociatedEntity
Name
Martin Calvino-Torterolo
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
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windows xp
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