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Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans

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TitleInfo
Title
Identification and characterization of components of rab-6-mediated trafficking in caenorhabditus elegans
Name (type = personal)
NamePart (type = family)
Sanner
NamePart (type = given)
James William
NamePart (type = date)
1987-
DisplayForm
James William Sanner
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Padgett
NamePart (type = given)
Richard
DisplayForm
Richard Padgett
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Firestein
NamePart (type = given)
Bonnie
DisplayForm
Bonnie Firestein
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Grant
NamePart (type = given)
Barth
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Barth Grant
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Rongo
NamePart (type = given)
Christopher
DisplayForm
Christopher Rongo
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2014
DateOther (qualifier = exact); (type = degree)
2014-10
Place
PlaceTerm (type = code)
xx
CopyrightDate (encoding = w3cdtf)
2014
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Membrane trafficking in neurons is an important mechanism used to regulate signaling. Controlling the abundance of AMPA-type receptors at the synapse is important for synaptic plasticity and influences processes involved in learning, memory formation, and motor control (Greger and Esteban, 2007; Shepherd and Huganir, 2007). In Caenorhabditis elegans, recycling of the AMPA receptor subunit GLR-1 has been demonstrated to be one method by which synaptic signaling mechanisms can be controlled. Rab GTPases 6.1 and 6.2 have been profiled for their role in trafficking of GLR-1 in neurons. RAB-6.2 plays a role in the retrograde trafficking of the AMPA-type glutamate receptor GLR-1 (Zhang et al., 2012). Activated Rab GTPases regulate membrane trafficking by recruiting multiple effectors, including proteins that modify phospholipid membrane composition, motor proteins that tether membranes to the cytoskeleton, and scaffolding proteins that bind to specific proteins within membranes (Stenmark, 2009). In order to understand RAB-6.2 function, we used a yeast two-hybrid approach to screen for candidate effector molecules. Herein, we discuss the screen performed and detail candidates of interest. We identified one particularly compelling candidate, a phosphoinositol-5-phosphatase named SAC-2, which we hypothesize to be involved in vesicle uncoating and/or in modifying membrane phospholipids. SAC-2::GFP is localized to punctate structures along the ventral nerve cord and in the neuron soma. SAC-2::GFP localization to puncta in the soma is enhanced in rab-6.2(ok2254) null mutant animals, which is opposite to our expectation for RAB-6.2 retrograde cargo or effector molecules. Additionally, we assessed the overlap in functions between RAB-6.1 and RAB-6.2, and delineating their pathways. In agreement with previous data from our lab, our findings suggest that the two RAB-6 isoforms perform similar trafficking functions, but do so independently of each other.
Subject (authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject (authority = ETD-LCSH)
Topic
Glutamic acid--Receptors
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5715
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (vii, 41 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by James William Sanner
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3CF9RRC
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Sanner
GivenName
James
MiddleName
William
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-06-18 22:52:01
AssociatedEntity
Name
James Sanner
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2016-10-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2016.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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