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Mechanisms of non-enveloped viral exit from the host

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TitleInfo
Title
Mechanisms of non-enveloped viral exit from the host
Name (type = personal)
NamePart (type = family)
Chen
NamePart (type = given)
Ying-Han
NamePart (type = date)
1977-
DisplayForm
Ying-Han Chen
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Cali
NamePart (type = given)
Ann
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Ann Cali
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Altan-Bonnet
NamePart (type = given)
Nihal
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Nihal Altan-Bonnet
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Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Friedman
NamePart (type = given)
Wilma
DisplayForm
Wilma Friedman
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Dobrowolski
NamePart (type = given)
Radek
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Radek Dobrowolski
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Cuervo
NamePart (type = given)
Ana Maria
DisplayForm
Ana Maria Cuervo
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - Newark
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2014
DateOther (qualifier = exact); (type = degree)
2014-10
CopyrightDate (encoding = w3cdtf)
2014
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Viral exit from the host cell is a critical step of the viral lifecycle. Enveloped viruses have employed numerous mechanisms to exit their host including direct budding out of the plasma membrane, budding into the secretory pathway to be trafficked out, or budding into the endosomal membrane system to be exocytosed. Comparatively little is known about how non-enveloped RNA viruses such as Poliovirus (PV), Coxsackievirus B3 (CVB3), Rotavirus, Reovirus exit the host cell. Here I showed PV hijacks and diverts the host autophagy pathway to capture numerous virions in autophagosomes which are then trafficked by actin machinery to filopodial extensions and fuse with the plasma membrane to release infectious large vesicles containing mature polio virions. I also demonstrated the infectivity of the virus when inside a vesicle is higher then when free likely due to the vesicles containing many virions as the “clustered bombs”. Finally, I found the exported viral vesicles are highly enriched in phosphatidylserine lipids and that the infection on subsequent host cells is dependent on not only the poliovirus receptor but also the phosphotidylserine lipids on the vesicles. My findings suggest that non-enveloped viruses also carry a piece of the host with them on their pathogenic journey. The lipid and protein components within these membranes potentially modulate the pathogenesis of non-enveloped viruses within the host and provide a new paradigm for viral spread and tropism.
Subject (authority = RUETD)
Topic
Biology
Subject (authority = ETD-LCSH)
Topic
Viruses
Subject (authority = ETD-LCSH)
Topic
Virology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_5781
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (viii, 117 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = vita)
Includes vita
Note (type = statement of responsibility)
by Ying-Han Chen
RelatedItem (type = host)
TitleInfo
Title
Graduate School - Newark Electronic Theses and Dissertations
Identifier (type = local)
rucore10002600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3SQ920Q
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Chen
GivenName
Ying-Han
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-08-17 11:32:04
AssociatedEntity
Name
YING-HAN CHEN
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - Newark
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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