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Whole genome expression in mice containing a human mannose binding gene (hMBL)
Descriptive
TitleInfo
Title
Whole genome expression in mice containing a human mannose binding gene (hMBL)
SubTitle
examining the immunological role of mAb 3F8 in mediating myocardial ischemia-reperfusion injuries
Name
(type = personal)
NamePart
(type = family)
Gorsuch
NamePart
(type = given)
William Brian
NamePart
(type = date)
1968-
DisplayForm
William Brian Gorsuch
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Stahl
NamePart
(type = given)
Greg L.
DisplayForm
Greg L. Stahl
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Shibata
NamePart
(type = given)
Masayuki
DisplayForm
Masayuki Shibata
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
co-chair
Name
(type = personal)
NamePart
(type = family)
Mahon
NamePart
(type = given)
Gwendolyn M.
DisplayForm
Gwendolyn M. Mahon
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Quackenbush
NamePart
(type = given)
John
DisplayForm
John Quackenbush
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
School of Health Related Professions
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2014
DateOther
(qualifier = exact);
(type = degree)
2014-10
Place
PlaceTerm
(type = code)
xx
CopyrightDate
(encoding = w3cdtf)
2014
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
During a myocardial ischemic event, acute occlusion sets in motion cell necrosis and myocardial tissue injury referred to as a myocardial ischemia-reperfusion (MI/R) injury. The resultant injury is triggered by an immunological response of which a major contributor involves the complement cascade of innate immune system involving mannose binding lectin (MBL). Few anti-complement therapeutics however have been approved for clinical use. Those studies to date have involved extensive whole genome expression in murine models of MI/R injury to assist in drug target elucidation. Studies performed have examined genomic traits and expression of mouse MBL (mMBL), which is not one hundred percent homologous to human mannose binding lectin (hMBL). In this study, novel hMBL+/+ mice treated with a novel mAb 3F8 were protected from MI/R injury as measured by area at risk and myocardial infarct staining when compared to control mice. Whole genome expression with the use of microarray was performed between hMBL mice undergoing MI/R treated with either a novel recombinant mAb 3F8 or mAb 1C10 as control. Mice treated with mAb 3F8 compared to mice treated with 1C10 revealed a significant down regulation in uncharacterized genes of the lncRNA family. Molecular modeling was used to study the three dimensional structural characteristics of mAb 3F8 recognition of hMBL. Within the hinge region of hMBL three possible locations were identified for the mAb 3F8 epitope for hMBL. These structurally similar locations offer possible insight into the ability of mAb 3F8 in protecting against MI/R injuries. These findings will assist in better understanding the genomic role hMBL in MI/R, the ability of a novel murine mAb 3F8 to modulate those effects and aide in continued drug target elucidation.
Subject
(authority = RUETD)
Topic
Biomedical Informatics
Subject
(authority = ETD-LCSH)
Topic
Coronary heart disease
Subject
(authority = ETD-LCSH)
Topic
Ischemia--Treatment
Subject
(authority = ETD-LCSH)
Topic
Reperfusion injury
Subject
(authority = ETD-LCSH)
Topic
Monoclonal antibodies--Therapeutic use
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_5873
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xiii, 97 p. : ill.)
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by William Brian Gorsuch
RelatedItem
(type = host)
TitleInfo
Title
School of Health Related Professions ETD Collection
Identifier
(type = local)
rucore10007400001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3PV6N02
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Gorsuch
GivenName
William
MiddleName
Brian
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2014-09-22 09:53:09
AssociatedEntity
Name
william Gorsuch
Role
Copyright holder
Affiliation
Rutgers University. School of Health Related Professions
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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Statistical Profile