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Simultaneous analyte quantification in low volume samples using a microfluidic device

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TitleInfo
Title
Simultaneous analyte quantification in low volume samples using a microfluidic device
Name (type = personal)
NamePart (type = family)
Ghodbane
NamePart (type = given)
Mehdi
NamePart (type = date)
1985-
DisplayForm
Mehdi Ghodbane
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Yarmush
NamePart (type = given)
Martin l
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Martin l Yarmush
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Schloss
NamePart (type = given)
Rene S
DisplayForm
Rene S Schloss
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Maguire
NamePart (type = given)
Tim J
DisplayForm
Tim J Maguire
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Zahn
NamePart (type = given)
Jeffrey D
DisplayForm
Jeffrey D Zahn
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Ramachandran
NamePart (type = given)
Rohit R
DisplayForm
Rohit R Ramachandran
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-01
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Immunoassays are one of the most widely performed assays in clinical and research settings due to their sensitivity, specificity, and ability to measure wide ranges of analytes. Recently, immunoassay technology has greatly improved due to the development of multiplex platforms, capable of measuring multiple analytes in a single sample. However, immunoassays are costly, time-consuming, and require relatively large sample volumes that inhibit their use in specific applications. Performing immunoassays using microfluidic devices has been shown to significantly reduce assay time, cost, and sample and reagent consumption. However, previous immunoassay devices possess drawbacks that prevent their broad use, including: low sensitivity, limited dynamic range, inability to change the analyte specificity, specialized reagent requirements, inability to produce quantitative data, and low sample throughput. Therefore, the objective of this dissertation was to develop a microfluidic immunoassay device overcoming the aforementioned limitations. A proof-of-concept device was developed capable of performing 8 parallel immunoassays on commercially available antibody conjugated microbeads. This eliminates the need for specialized reagents while allowing any analyte, for which antibodies are available, to be measured. Furthermore, we developed the first experimentally validated computational fluid dynamic model of antibody antigen binding in microchannels. Design of experiments (DOE) and multi-objective optimization techniques were used in conjunction with the model to optimize an IL-6 immunoassay with a sensitivity of 358 fM using only 1.35 μL of sample volume. The device design was then scaled-up to allow 32 samples to be processed simultaneously. With the expanded device, we demonstrated high-sensitivity, a large dynamic range, and quantification of 6 cytokines (Il-1b, Il-6, IL-10, IL-13, MCP-1, and TNF-a). Finally, we measured in vitro experimental supernatants in parallel using the microdevice and a conventional benchtop assay. The microdevice provided comparable results while reducing sample volume from 50 to 4.2 μL. In summary, we demonstrated a low-volume, highly sensitive assay with a large dynamic range capable of processing large numbers of samples using commercially available reagents. Due to these advantages, the technology in this work has far-reaching in vitro, in vivo, and clinical applications.
Subject (authority = RUETD)
Topic
Biomedical Engineering
Subject (authority = ETD-LCSH)
Topic
Microfluidics
Subject (authority = ETD-LCSH)
Topic
Immunoassay--Methods
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6084
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xvi, 147 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Mehdi Ghodbane
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T370835S
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Ghodbane
GivenName
Mehdi
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2014-12-18 17:04:58
AssociatedEntity
Name
Mehdi Ghodbane
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-01-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2017-01-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after January 30th, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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ETD
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windows xp
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