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Customization and optimization of a histone h2a-based vector for targeted gene transfer to cancer cells
Descriptive
TitleInfo
Title
Customization and optimization of a histone h2a-based vector for targeted gene transfer to cancer cells
Name
(type = personal)
NamePart
(type = family)
Karjoo Diarkhan
NamePart
(type = given)
Zahra
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1980-
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Zahra Karjoo Diarkhan
Role
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author
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Hatefi
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Arash
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Arash Hatefi
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Advisory Committee
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chair
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Minko
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Tamara
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Tamara Minko
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Advisory Committee
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internal member
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(type = personal)
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You
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(type = given)
Guofeng
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Guofeng You
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Advisory Committee
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(authority = RULIB)
internal member
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GOW
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(type = given)
ANDREW
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ANDREW GOW
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Advisory Committee
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outside member
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Rutgers University
Role
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(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
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school
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Text
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theses
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2015
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(type = degree)
2015-01
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2015
Place
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xx
Language
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eng
Abstract
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Developing an efficient and safe system for gene delivery is considered the bottleneck of gene therapy, where a successful delivery of the nucleic acid can reverse a defective cellular pathway to normal, eradicate cancer at molecular level or simply make it more susceptible to current chemotherapies. Not only have the intracellular events played a crucial role for obtaining a successful gene delivery, but the interaction of nano-particles with extracellular factors should be studied as well. The goal of this study was to design, produce and optimize a non-viral gene delivery system for targeted delivery of nucleic acid such as reporter genes (e.g., green fluorescent protein) or therapeutic genes (e.g., suicide genes) to cancer cells. The system was designed in a way to be easily customized for different cancer types, still presenting a high level of targeted delivery. The first chapter of this thesis will focus on the concept of gene therapy and current systems used for this modality. Different types of vectors including viral and non-viral polymeric vectors will be discussed briefly and the advantages and disadvantages of each will be mentioned. We also discussed natured inspired biopolymers such as peptides and amino acid based vectors which are the fundamental premise of this study. In chapter II, the concept of suicide gene therapy will be explained. Additionally, the current enzyme/prodrug systems, different methods for delivery of suicide genes will be elaborated. In chapter III and IV, the new nanotechnology platform for targeted delivery of plasmid DNA to HER2-positive ovarian cancer cells and HER2-negative prostate cancer cells will be presented. In chapter III, the method for optimization of nanotechnology platform will be discussed and the features of optimized particles will be presented. Chapter IV explains the modification we introduced to the vectors’ primary structure to customize it for a different cell line. Also another method for optimization of amino-acid based vectors for in vivo delivery will be introduced. In these two chapters, the methods of designing and the efficiency of each vector to fulfill the expected goals as well as their safety will be discussed in details and supportive data will be presented.
Subject
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Topic
Pharmaceutical Science
RelatedItem
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Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
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ETD_6045
PhysicalDescription
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (xii, 157 p. : ill.)
Note
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Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Gene therapy
Subject
(authority = ETD-LCSH)
Topic
Cancer--Treatment
Subject
(authority = ETD-LCSH)
Topic
Nanoparticles
Note
(type = statement of responsibility)
by Zahra Karjoo Diarkhan
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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NjNbRU
Identifier
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doi:10.7282/T37S7QGT
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Karjoo Diarkhan
GivenName
Zahra
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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2014-12-07 18:27:11
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Name
Zahra Karjoo Diarkhan
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Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Type
License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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2015-01-31
DateTime
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2017-01-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after January 30th, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
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ETD
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windows xp
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