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S-Nitrosylation in Organs of Mice Exposed to Low or High Doses of γ-Rays: The Modulating Effect of Iodine Contrast Agent at a Low Radiation Dose

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TypeOfResource
Text
TitleInfo
Title
S-Nitrosylation in Organs of Mice Exposed to Low or High Doses of γ-Rays: The Modulating Effect of Iodine Contrast Agent at a Low Radiation Dose
Name (type = personal)
NamePart (type = family)
Nicolas
NamePart (type = given)
Fadia
Affiliation
Health Informatics, Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Wu
NamePart (type = given)
Changgong
Affiliation
Biochemistry and Molecular Biology, Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Bukhari
NamePart (type = given)
Salwa
Affiliation
Radiology, Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
de Toledo
NamePart (type = given)
Sonia M.
Affiliation
Radiology, Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Li
NamePart (type = given)
Hong
Affiliation
Biochemistry and Molecular Biology, Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0002-8125-4971)
NamePart (type = family)
Shibata
NamePart (type = given)
Masayuki
Affiliation
Health Informatics, Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Azzam
NamePart (type = given)
Edouard I.
Affiliation
Radiology (New Jersey Medical School, NJMS), Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Radiology (New Jersey Medical School, NJMS)
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Health Informatics
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Biochemistry and Molecular Biology
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
New Jersey Medical School (NJMS)
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
Rutgers School of Health Related Professions (Rutgers SHRP)
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Version of Record (VoR)
Note (type = peerReview)
Peer reviewed
OriginInfo
Publisher
MDPI
DateIssued (encoding = w3cdtf); (qualifier = exact); (keyDate = yes)
2015
Abstract (type = Abstract)
The covalent addition of nitric oxide (NO•) onto cysteine thiols, or S-nitrosylation, modulates the activity of key signaling proteins. The dysregulation of normal S-nitrosylation contributes to degenerative conditions and to cancer. To gain insight into the biochemical changes induced by low-dose ionizing radiation, we determined global S-nitrosylation by the “biotin switch” assay coupled with mass spectrometry analyses in organs of C57BL/6J mice exposed to acute 0.1 Gy of cesium-137 γ-rays. The dose of radiation was delivered to the whole body in the presence or absence of iopamidol, an iodinated contrast agent used during radiological examinations. To investigate whether similar or distinct nitrosylation patterns are induced following high-dose irradiation, mice were exposed in parallel to acute 4 Gy of cesium-137 γ rays. Analysis of modulated S-nitrosothiols (SNO-proteins) in freshly-harvested organs of animals sacrificed 13 days after irradiation revealed radiation dose- and contrast agent-dependent changes. The major results were as follows: (i) iopamidol alone had significant effects on S-nitrosylation in brain, lung and liver; (ii) relative to the control, exposure to 0.1 Gy without iopamidol resulted in statistically-significant SNO changes in proteins that differ in molecular weight in liver, lung, brain and blood plasma; (iii) iopamidol enhanced the decrease in S-nitrosylation induced by 0.1 Gy in brain; (iv) whereas a decrease in S-nitrosylation occurred at 0.1 Gy for proteins of ~50 kDa in brain and for proteins of ~37 kDa in liver, an increase was detected at 4 Gy in both organs; (v) mass spectrometry analyses of nitrosylated proteins in brain revealed differential modulation of SNO proteins (e.g., sodium/potassium-transporting ATPase subunit beta-1; beta tubulins; ADP-ribosylation factor 5) by low- and high-dose irradiation; and (vi) ingenuity pathway analysis identified major signaling networks to be modulated, in particular the neuronal nitric oxide synthase signaling pathway was differentially modulated by low- and high-dose γ-irradiation.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
18 p.
Subject (authority = local)
Topic
Ionizing radiation
Subject (authority = local)
Topic
Radiation sensitivity
Subject (authority = local)
Topic
S-nitrosylation
Subject (authority = local)
Topic
Low- and high-dose γ ray responses
Subject (authority = local)
Topic
Radiodiagnostic procedures
Subject (authority = local)
Topic
Network analyses in brain
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2015
AssociatedObject
Name
Proteomes
Type
Journal
Relationship
Has part
Detail
56-73
Identifier (type = volume and issue)
3(2)
Reference (type = url)
http://www.mdpi.com/2227-7382/3/2/56
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
National Institutes of Health
AssociatedEntity
Role
Originator
Name
Edouard Azzam
AssociatedObject
Type
Grant number
Name
CA049062 (Sub # 5443)
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
New Jersey Health Foundation
AssociatedEntity
Role
Originator
Name
Masayuki Shibata
AssociatedObject
Type
Grant number
Name
PC27-15
RelatedItem (type = host)
TitleInfo
Title
Azzam, Edouard I.
Identifier (type = local)
rucore30163800001
RelatedItem (type = host)
TitleInfo
Title
De Toledo, Sonia M.
Identifier (type = local)
rucore30164000001
RelatedItem (type = host)
TitleInfo
Title
Li, Hong
Identifier (type = local)
rucore30164100001
RelatedItem (type = host)
TitleInfo
Title
Shibata, Masayuki
Identifier (type = local)
rucore30137800001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3DZ0B3Z
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