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Identifying the molecular mechanism and genetic region for habitat specific clock variation in N.discreta PS4B

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TitleInfo
Title
Identifying the molecular mechanism and genetic region for habitat specific clock variation in N.discreta PS4B
Name (type = personal)
NamePart (type = family)
Pachucki
NamePart (type = given)
Ryan J.
DisplayForm
Ryan J. Pachucki
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Lee
NamePart (type = given)
Kwangwon
DisplayForm
Kwangwon Lee
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Shende
NamePart (type = given)
Sunil
DisplayForm
Sunil Shende
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Klein
NamePart (type = given)
Eric
DisplayForm
Eric Klein
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Martin
NamePart (type = given)
Joseph
DisplayForm
Joseph Martin
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Camden Graduate School
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-05
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
The 24-hour biological rhythm, or circadian rhythm, has been attributed as a fitness trait in multiple organisms. [1][2][3] To identify how organisms adapted their circadian rhythms to increase their fitness, we used a global population from the Neurospora discreta complex. Under cycling light conditions, North American strains in N. discreta PS4B exhibit no rhythms in their sporulation output. To understand the molecular variation of the oscillator underlying these divergent phenotypes, we analyzed the expression of the key clock protein FREQUENCY and found FRQ levels have a rhythm in constant conditions. Based on our findings, we concluded that the North American strains in the N. discreta PS4B population have decoupled their developmental rhythm from their molecular oscillator to enhance their fitness. Our mathematical model supports the hypothesis that North American strains have increased their fitness by decreasing their coupling coefficient and threshold. In addition, we found the candidate region for habitat specific clock variation (HSCV) occurs on chromosome 3. In an intercontinental F1 population, we observed an opposite allele effect, resulting in strains with the North American phenotype having the African parent allele. To understand the mechanism and components of another oscillator, we used the strain PRD-1, which is hypothesized in the literature to be a key component of the metabolic oscillator. We have observed arrhythmic ATP oscillation in PRD-1 compared to WT strains.
Subject (authority = RUETD)
Topic
Biology
Subject (authority = ETD-LCSH)
Topic
Circadian rhythms
Subject (authority = ETD-LCSH)
Topic
Neurospora
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6503
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (vii, 31 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Ryan J. Pachucki
RelatedItem (type = host)
TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
Identifier (type = local)
rucore10005600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T37P9174
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Pachucki
GivenName
Ryan
MiddleName
J.
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-05-01 11:47:00
AssociatedEntity
Name
Ryan Pachucki
Role
Copyright holder
Affiliation
Rutgers University. Camden Graduate School
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Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2017-05-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 30th, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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