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A study on the effects of triterpenoids on prostate cancer prevention
Descriptive
TitleInfo
Title
A study on the effects of triterpenoids on prostate cancer prevention
Name
(type = personal)
NamePart
(type = family)
Al-Mahmood
NamePart
(type = given)
Asia Abed
NamePart
(type = date)
1984-
DisplayForm
Asia Abed Al-Mahmood
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Kong
NamePart
(type = given)
Ah-Ng Tony
DisplayForm
Ah-Ng Tony Kong
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Kagan
NamePart
(type = given)
Leonid
DisplayForm
Leonid Kagan
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Hatefi
NamePart
(type = given)
Arash
DisplayForm
Arash Hatefi
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2015
DateOther
(qualifier = exact);
(type = degree)
2015-05
Place
PlaceTerm
(type = code)
xx
CopyrightDate
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(qualifier = exact)
2015
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Prostate cancer is the one of the most common cancers in the world, and the incidence of this disease increases annually. Many phytochemicals have been shown to be potent inhibitors in preventing prostate cancer disease initiation, progression or inhibit metastasis. Cancer chemoprevention by relatively non-toxic commonly consumed dietary phytochemicals or synthetic compounds would be a logical approach in reducing cancer incidence, and progression to advanced-resistant cancer cells. Synthetic oleanane triterpenoids have been shown to be effective compounds in prostate cancer chemoprevention. The nuclear E2-factor related factor 2 (Nrf2) is the master regulator of many detoxifying and antioxidant enzymes. It is considered the most important chemopreventive signaling pathway in inhibiting cancer initiation and progression. In this study, two synthetic triterpenoids CDDO-Im and CDDO-EA were used to investigate their effects on the activation of Nrf2 and Nrf2-mediated downstream target genes. These compounds were first tested on HepG2-C8 cells stably transfected with ARE-luciferase reporter gene, for cell toxicity and their potential to induce the Nrf2/ARE pathway. CDDO-Im and CDDO-EA were found to could induce ARE-luciferase activities at relatively low and non-toxic concentrations. Using quantitative PCR (qPCR) and western blotting, the transcription of Nrf2 downstream genes HO-1 and SOD1 were significantly induced by drug treatments, corresponding with the increase in their protein levels in human prostate LNCaP cells. These results suggest that CDDO-Im and CDDO-EA could elicit their chemopreventive effect via the activation of Nrf2 pathway. Emerging evidence suggests epigenetic mechanism such as DNA methylation and histone modifications could potentially be involved in the regulation of Nrf2 pathway. In this study, the effects of CDDO-Im and CDDO-EA on histone deacetylases (HDAC) were investigated by using qPCR. Both compounds show inhibitory effects on HDAC1, HDAC2 and HDAC3 which are considered the highly expressed type of HDACs in prostate cancer. Both compounds can also inhibit HDAC6 at low concentrations. In summary, this study shows that CDDO-Im and CDDO-EA are potentially interesting compounds for prostate cancer chemoprevention via activating Nrf2/ARE signaling pathway as well as epigenetic regulation.
Subject
(authority = RUETD)
Topic
Pharmaceutical Science
Subject
(authority = ETD-LCSH)
Topic
Prostate--Cancer--Prevention
Subject
(authority = ETD-LCSH)
Topic
Cancer--Chemoprevention
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_6439
PhysicalDescription
Form
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electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (ix, 76 p. : ill.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Asia Abed Al-Mahmood
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3ZS2ZBJ
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Al-Mahmood
GivenName
Asia
MiddleName
Abed
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2015-04-17 14:53:12
AssociatedEntity
Name
Asia Al-Mahmood
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2015-05-31
DateTime
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2016-05-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 30th, 2016.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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