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Characterizing chronic lymphocytic leukemia for novel biomarkers of CD40 responsiveness using intracellular flow cytometry
Descriptive
TitleInfo
Title
Characterizing chronic lymphocytic leukemia for novel biomarkers of CD40 responsiveness using intracellular flow cytometry
Name
(type = personal)
NamePart
(type = family)
Boody
NamePart
(type = given)
Christopher M.
NamePart
(type = date)
1989-
DisplayForm
Christopher M. Boody
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Covey
NamePart
(type = given)
Lori R
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Lori R Covey
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Denzin
NamePart
(type = given)
Lisa
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Lisa Denzin
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
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Xie
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Ping
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Ping Xie
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
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2015
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2015-05
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2015
Place
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xx
Language
LanguageTerm
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(type = code)
eng
Abstract
(type = abstract)
Chronic Lymphocytic Leukemia (CLL) is the most common form of leukemia. CLL is characterized by a slow progression that generally worsens over time and currently is without a cure. A number of prognostic indicators are currently utilized to identify CLLs with a more progressive disease course. One such prognostic indicator is the responsiveness of the CLL to CD40 stimulation. CLLs that do not demonstrate a functional response to CD40L show a more aggressive clinical outcome than those that are responsive. We sought to establish a system to classify CLL cases as CD40 responsive or unresponsive using novel biomarkers by intracellular flow cytometry. This allowed us to analyze a very small number of cells from each CLL. Altogether, our work addressed optimizing in vitro culturing conditions for proliferation and viability. Also, this work analyzed a number of CD40 stimulation conditions to determine the optimal approach to analyzing CD40-mediated events in signaling. Finally, this established a system to evaluate early intracellular signaling in the NF-κB canonical pathway with p65 phosphorylation and IκBα degradation as well as upregulation of CD80 following extended stimulation. Using IκBα we were able to discern CD40 activation in CLLs that were previously characterized as unresponsive with p65 and CD80 as markers. Thus, our work extends the definition of “CD40 responsiveness” and suggests that CD40-mediated CD80 responses, which are linked to co-stimulation, may be a better predictor of CLL prognostic outcomes than early NF-B responses.
Subject
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject
(authority = ETD-LCSH)
Topic
Cancer biology
Subject
(authority = ETD-LCSH)
Topic
CD antigens
Subject
(authority = ETD-LCSH)
Topic
Chronic lymphocytic leukemia
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_6338
PhysicalDescription
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (ix, 65 p. : ill.)
Note
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M.S.
Note
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Includes bibliographical references
Note
(type = statement of responsibility)
by Christopher M. Boody
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T35B04B7
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Boody
GivenName
Christopher
MiddleName
M.
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2015-04-13 14:00:39
AssociatedEntity
Name
Christopher Boody
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2015-05-31
DateTime
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(point = end)
2016-05-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 30th, 2016.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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Statistical Profile