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Characterization of anaerobic methyl tert-butyl ether (MTBE)-degrading communities
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Liu, Tong. Characterization of anaerobic methyl tert-butyl ether (MTBE)-degrading communities. Retrieved from https://doi.org/doi:10.7282/T31G0P3V

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TitleCharacterization of anaerobic methyl tert-butyl ether (MTBE)-degrading communities
NameLiu, Tong (author); Häggblom, Max M (chair); Kerkhof, Lee J (internal member); Fennel, Donna (internal member); Fahrenfeld, Nicole (outside member); Rutgers University; Graduate School - New Brunswick
Date Created2015
Other Date2015-05 (degree)
SubjectEnvironmental Sciences, Butyl methyl ether--Environmental aspects, Drinking water--Contamination
Extent1 online resource (viii, 109 p. : ill.)
DescriptionThe wide use of methyl tert-butyl ether (MTBE), a synthetic fuel oxygenate, have caused extensive contamination in groundwater in past two decades and resulted in taste, odor, and potential toxicity problems in drinking water. MTBE contaminated groundwater aquifer is often depleted of oxygen and microorganisms can anaerobically degraded MTBE under a verity of redox conditions. It has been demonstrated that MTBE can be degraded anaerobically which makes in situ bioremediation of contaminated aquifers a potential solution to address this problem. Assessment and enhancement of MTBE bioremediation requires knowledge of the microorganisms that responsible for biodegradation process. However, it is challenge to isolate anaerobic MTBE-degrading microorganisms or to characterize the microbial communities. The aim of this study was to identify the organisms that mediate anaerobic biodegradation of MTBE in methanogenic or sulfidogenic cultures enriched from estuarine sediments. Stable isotope probing (SIP) combined with terminal restriction fragment length polymorphism (T-RFLP) analysis showed that Ruminococcaceae species were active in methanogenic MTBE-degrading community. T-RFLP coupled with clone library analysis of bacterial 16S rRNA genes from sulfidogenic enrichment cultures showed Deltaproterobacteria were highly enriched than the other phylogenetically diverse populations. We also investigated MTBE-degrading communities by single cell using Raman Spectroscopy and SIP. The results confirmed the MTBE degraders are not abundant in the communities. This study provides crucial information for understanding the mechanisms of anaerobic degradation of MTBE as well as for assessment of the in situ bioremediation at contaminated field sites as the microbial/molecular tools.
NotePh.D.
NoteIncludes bibliographical references
Noteby Tong Liu
Genretheses, ETD doctoral
Persistent URLhttps://doi.org/doi:10.7282/T31G0P3V
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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