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Investigation of bioaerosol characterization bias due to aerosolization and sampling

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TitleInfo
Title
Investigation of bioaerosol characterization bias due to aerosolization and sampling
Name (type = personal)
NamePart (type = family)
Zhen
NamePart (type = given)
Huajun
NamePart (type = date)
1985-
DisplayForm
Huajun Zhen
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Mainelis
NamePart (type = given)
Gediminas
DisplayForm
Gediminas Mainelis
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Fennell
NamePart (type = given)
Donna E
DisplayForm
Donna E Fennell
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Reinfelder
NamePart (type = given)
John
DisplayForm
John Reinfelder
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
SCHWANDER
NamePart (type = given)
STEPHAN
DisplayForm
STEPHAN SCHWANDER
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-05
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Bioaerosols have been investigated for their adverse effects on human health and also their roles in cloud formation, precipitation and atmospheric chemical reactions. In these studies, it is often necessary to collect biological cells from the airborne state or to disperse the cells into the air using a variety of sampling and aerosolization devices. These devices inevitably exert stress on the cells and result in changes in their biological characteristics, e.g. loss of culturability, impairment of cell membrane, and change in cellular activities. It is highly likely that after experiencing such stress, collected microorganisms are not representative of cells prior to sampling and aerosolization, and thus could introduce biases in their characterization. In this dissertation, the potential bioaerosol characterization biases induced by several commonly used sampling and aerosolization devices were investigated. The stress experienced by E. coli during aerosolization was found to depend on a particular aerosolization device. Particularly, a newly developed pneumatic nebulizer, the Single-Pass Aerosolizer, was shown to better preserve cell culturability and membrane integrity compared to the commonly used Collison nebulizer at similar biological particles output concentrations. Several bioaerosol samplers that employ filtration, impingement, impaction and electrostatic precipitation for sample collection were tested with respect to their effects on the cell membrane integrity and cellular 16S rRNA content of Escherichia coli cells. Sampling stress resulted in severe membrane impairment to E. coli aerosols, leading to the release of genomic DNA as extracellular molecules. Extracellular DNA should be taken into account when analyzing bioaerosol samples to more accurately quantify bacterial presence. Cell membrane damage to bioaerosols depended on which sampler was used and could be reduced by modifying specific operational parameters. E. coli cells exhibited variation in 16S ribosomal RNA (rRNA) level when exposed to long-term air sampling in laboratory experiment, suggesting a change of biological activity in response to sampling stress. The importance of this effect for those taxa in airborne bacterial community from a variety of environments should be examined. The abundance of bacterial 16S rRNA in bioaerosols collected from an outdoor environment was about two orders of magnitude higher than that of 16S rRNA gene. However, the sampler-dependent bias effect on analysis of 16S rRNA sequences for bacterial community composition was minimal in investigated outdoor bioaerosols.
Subject (authority = RUETD)
Topic
Environmental Sciences
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6036
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xiii, 206 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
Biosensors
Subject (authority = ETD-LCSH)
Topic
Aerosols--Measurement
Note (type = statement of responsibility)
by Huajun Zhen
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3S75J6V
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Zhen
GivenName
Huajun
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-03-14 10:54:50
AssociatedEntity
Name
Huajun Zhen
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2017-05-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 30th, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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