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A study of retrograde transport using Caenorhabditis elegans intestinal cells as a model

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Title
A study of retrograde transport using Caenorhabditis elegans intestinal cells as a model
Name (type = personal)
NamePart (type = family)
Bai
NamePart (type = given)
Zhiyong
NamePart (type = date)
1982-
DisplayForm
Zhiyong Bai
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Barr
NamePart (type = given)
Maureen
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Maureen Barr
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Grant
NamePart (type = given)
Barth
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Barth Grant
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
co-chair
Name (type = personal)
NamePart (type = family)
Rongo
NamePart (type = given)
Christopher
DisplayForm
Christopher Rongo
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Kramer
NamePart (type = given)
Sunita
DisplayForm
Sunita Kramer
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Endocytosis mediates the internalization of cell surface proteins and lipids in small vesicles that bud from the plasma membrane and deliver their cargo to endosomes. When cargo proteins reach the endosomes, one important pathway that they may follow is retrograde transport, in which cargos are delivered from endosomes to the Golgi apparatus. The role of the recycling endosome in retrograde transport has not been studied well in the past. We use C. elegans, especially the intestinal cells, as model to study retrograde trafficking, employing a combination of genetics, cell biology, biochemistry and molecular biology tools. We discovered that CDC-42/TOCAs/PARs/WAVE localize to the recycling endosome, and regulate retrograde transport at a step from Recycling endosome to Trans-Golgi Network. When missing functional CDC-42/TOCAs/PARs/WAVE, MIG-14/Wntless is misdirected to the late endosome/lysosome during post-endocytic sorting, and steady-state MIG-14 protein levels are strongly reduced. Neuronal polarity defects in mechanosensory neurons ALM are also observed under these conditions, consistent with a defect in MIG-14-dependent WNT signaling. Interestingly, we also found that mutants lacking retromer components SNX-3, VPS-35, VPS-26, or VPS-29, that aberrantly degrade MIG-14 during retrograde transport, also aberrantly degrade the human Transferrin receptor during early endosome to recycling endosome transport. Furthermore, we tested TGN-38, the worm homolog of a well known retrograde cargo TGN38 that passes through the recycling endosome. When missing functional CDC-42, TGN-38 is mainly trapped at the recycling endosome, while upon loss of functional SNX-3, TGN-38 is trapped at the early endosome. These results suggest CDC-42/TOCAs/PARs/WAVE regulate retrograde transport at a step from Recycling endosome to the Trans-Golgi Network after SNX-3/VPS-35/VPS-26/VPS-29, which regulates early endosome to recycling endosome transport. Lastly, we obtained evidence that CDC-42/TOCAs/PARs/WAVE complex may use clathrin/AP-1 vesicles, and that OCRL-1/CKII may work with CDC-42/TOCAs/PARs/WAVE and regulate retrograde transport from the recycling endosome to Trans-Golgi Network. We hope that our work will shed light on the study of intracellular trafficking, especially retrograde transport.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (authority = ETD-LCSH)
Topic
Caenorhabditis elegans
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6617
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xiii, 112 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Zhiyong Bai
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3GQ70R6
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Bai
GivenName
Zhiyong
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-07-31 12:58:58
AssociatedEntity
Name
zhiyong bai
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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License
Name
Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
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Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
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ETD
OperatingSystem (VERSION = 5.1)
windows xp
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