Staff View
An RNA interference knock-down of nitrate reductase enhances lipid biosynthesis in the diatom Phaeodactylum tricornutum

Descriptive

TypeOfResource
Text
TitleInfo
Title
An RNA interference knock-down of nitrate reductase enhances lipid biosynthesis in the diatom Phaeodactylum tricornutum
Name (type = personal)
NamePart (type = family)
Levitan
NamePart (type = given)
Orly
Affiliation
Marine and Coastal Sciences, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Dinamarca
NamePart (type = given)
Jorge
Affiliation
Marine and Coastal Sciences, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Zelzion
NamePart (type = given)
Ehud
Affiliation
Ecology, Evolution and Natural Resources, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Gorbunov
NamePart (type = given)
Maxim Y.
Affiliation
Marine and Coastal Sciences, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Falkowski
NamePart (type = given)
Paul G.
Affiliation
Marine and Coastal Sciences, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Environmental and Biological Sciences (SEBS)
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Marine and Coastal Sciences
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Ecology, Evolution and Natural Resources
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
Note (type = peerReview)
Peer reviewed
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2015
Publisher
John Wiley & Sons Ltd
Abstract (type = Abstract)
When diatoms are stressed for inorganic nitrogen they remodel their intermediate metabolism and redirect carbon towards lipid biosynthesis. However, this response comes at a significant cost reflected in decreased photosynthetic energy conversion efficiency and growth. Here we explore a molecular genetics approach to restrict the assimilation of inorganic nitrogen by knocking down nitrate reductase (NR). The transformant strain, NR21, exhibited about 50% lower expression and activity of the enzyme but simultaneously accumulated over 40% more fatty acids. However, in contrast to nitrogen-stressed wild-type (WT) cells, which grow at about 20% of the rate of nitrogen-replete cells, growth of NR21 was only reduced by about 30%. Biophysical analyses revealed that the photosynthetic energy conversion efficiency of photosystem II was unaffected in NR21; nevertheless, the plastoquinone pool was reduced by 50% at the optimal growth irradiance while in the WT it was over 90% oxidized. Further analyses reveal a 12-fold increase in the glutamate/glutamine ratio and an increase NADPH and malonyl-CoA pool size. Transcriptomic analyses indicate that the knock down resulted in changes in the expression of genes for lipid biosynthesis, as well as the expression of specific transcription factors. Based on these observations, we hypothesize that the allocation of carbon and reductants in diatoms is controlled by a feedback mechanism between intermediate metabolites, the redox state of the plastid and the expression and binding of transcription factors related to stress responses.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
46 p.
Subject (authority = local)
Topic
Phaeodactylum tricornutum
Subject (authority = local)
Topic
Nitrate reductase
Subject (authority = LCSH)
Topic
Lipids
Subject (authority = LCSH)
Topic
RNA interference
Subject (authority = LCSH)
Topic
Photosynthesis
Subject (authority = local)
Topic
GSE67449
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2015
AssociatedObject
Name
Plant Journal
Type
Journal
Relationship
Has part
Detail
963-973
Identifier (type = volume and issue)
84(5)
Reference (type = url)
http://dx.doi.org/10.1111/tpj.13052
RelatedItem (type = host)
TitleInfo
Title
Zelzion, Ehud
Identifier (type = local)
rucore30187300001
RelatedItem (type = host)
TitleInfo
Title
Gorbunov, Maxim Y.
Identifier (type = local)
rucore30187400001
RelatedItem (type = host)
TitleInfo
Title
Falkowski, Paul G.
Identifier (type = local)
rucore30187500001
RelatedItem (type = host)
TitleInfo
Title
Levitan, Orly
Identifier (type = local)
rucore30185500001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3NZ89MG
Back to the top

Rights

RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004); (TYPE = [FS] statement #1)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers. Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work. I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others. I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work. By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Publication notice
Detail
This is the peer reviewed version of the following article: Levitan, O., Dinamarca, J., Zelzion, E., Gorbunov, M. Y. and Falkowski, P. G. (2015), An RNA interference knock-down of nitrate reductase enhances lipid biosynthesis in the diatom Phaeodactylum tricornutum. Plant J, 84: 963–973., which has been published in final form at https://dx.doi.org/10.1111/tpj.13052. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
RightsEvent
Type
Embargo
DateTime (encoding = w3cdtf); (point = start)
2015-12-21
DateTime (encoding = w3cdtf); (point = end)
2016-12-07
Detail
Access to this PDF has been restricted at the publisher's request.The publisher, Wiley, requires a 12 month embargo from the date the article was published. The article in this repository will be publicly available after December 7, 2016.
RightsHolder (type = personal)
Name
FamilyName
Levitan
GivenName
Orly
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Dinamarca
GivenName
Jorge
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Zelzion
GivenName
Ehud
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Gorbunov
GivenName
Maxim
MiddleName
Y.
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Falkowski
GivenName
Paul
MiddleName
G.
Role
Copyright holder
Back to the top

Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
Back to the top
Version 8.3.10
Rutgers University Libraries - Copyright ©2019