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Analysis of human Aurora C Kinase variants in oocyte meiosis

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TitleInfo
Title
Analysis of human Aurora C Kinase variants in oocyte meiosis
Name (type = personal)
NamePart (type = family)
Fellmeth
NamePart (type = given)
Jessica
NamePart (type = date)
1983-
DisplayForm
Jessica Fellmeth
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Schindler
NamePart (type = given)
Karen
DisplayForm
Karen Schindler
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Mckim
NamePart (type = given)
Kim
DisplayForm
Kim Mckim
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Treff
NamePart (type = given)
Nathan
DisplayForm
Nathan Treff
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Uzumcu
NamePart (type = given)
Mehmet
DisplayForm
Mehmet Uzumcu
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
The birth of a healthy child is often a priority for couples, yet many couples will unfortunately suffer from infertility. Aneuploidy is the leading genetic abnormality that causes infertility. Aneuploidies in sperm and eggs arise from errors during chromosome segregation in meiosis. Though many of the critical players are known and mutants characterized, the regulatory components involved in directing accurate chromosome segregation are still not entirely clear. Aurora kinase C is one of the critial players in chromosomes segregation in meiosis and in this thesis, I establish the use of Aurkc-/- mouse oocytes as an effective model for studying human AURKC ex vivo. By comparing the rescue of the knockout phenotype when hAURKC and mAURKC are expressed in the oocytes, I show that hAURKC functions similarly in female meiosis as does mAURKC. I also characterized three splice variants of hAURKC in this model after establishing their simultaneous expression in human oocytes. The three splice variants have different abilities and stabilities in meiosis, but promote optimal chromosome segregation when expressed simultaneously during oocyte meiosis. Finally, I used the verified model to characterize the phenotype of three sterility-associated mutations in human AURKC. I show through functional assays that the three mutations have differing abilities in meiosis, but all result in a loss of function of the AURKC-CPC, which causes meiotic arrest and polyploid gametes. This work contributes to the field of reproductive biology by providing a tractable model for studying human AURKC and for further analysis of mutants and variants.
Subject (authority = RUETD)
Topic
Microbiology and Molecular Genetics
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6668
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xi, 160 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
Protein kinases
Subject (authority = ETD-LCSH)
Topic
Ovum
Note (type = statement of responsibility)
by Jessica Fellmeth
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3FB54X7
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Fellmeth
GivenName
Jessica
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-08-24 15:10:30
AssociatedEntity
Name
Jessica Fellmeth
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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