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Sponge-associated dehalogenating microorganisms and isotope analysis of their dehalogenation of brominated phenols
Descriptive
TitleInfo
Title
Sponge-associated dehalogenating microorganisms and isotope analysis of their dehalogenation of brominated phenols
Name
(type = personal)
NamePart
(type = family)
Gray
NamePart
(type = given)
Isabel Del Carmen
NamePart
(type = date)
1978-
DisplayForm
Isabel Del Carmen Gray
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Häggblom
NamePart
(type = given)
Max H
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Max H Häggblom
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Advisory Committee
Role
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chair
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(type = personal)
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(type = family)
Kerkhof
NamePart
(type = given)
Lee
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Lee Kerkhof
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Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Fennell
NamePart
(type = given)
Donna
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Donna Fennell
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Nijenhuis
NamePart
(type = given)
Ivonne
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Ivonne Nijenhuis
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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school
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Text
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theses
OriginInfo
DateCreated
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(qualifier = exact)
2015
DateOther
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(type = degree)
2015-10
CopyrightDate
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2015
Place
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(type = code)
xx
Language
LanguageTerm
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(type = code)
eng
Abstract
(type = abstract)
Sponges are filter feeders, ancient animals that have been extremely successful in surviving for over 600 million years almost unchanged. These metazoa can harbor microbes comprising almost 35% of their bodies at densities of 109 cells/g. Sponges are also rich in organohalides, usually with bioactive cytostatic and/or cytotoxic characteristics. Some of these organohalides resemble anthropogenic pollutants, such as halogenated dioxins and flame retardants. Thus, in nature some sponges contain both a high number of microbes as well as a high concentration of organohalides. This includes the sponge Aplysina aerophoba, from where Desulfoluna spongiiphila was isolated, a sulfate reducing Deltaproteobacteria that can reductively dehalogenate bromophenols. This observation led to the interest of enriching for sponge-associated microbes capable of dehalogenation from different ecoregions of the temperate and tropical oceans. The hypothesis is that dehalogenating bacteria are widespread among sponge species regardless of geographical location and form stable populations within the sponge animal that function in the cycling of organohalide compounds. Anaerobic dehalogenating activity was found to be widespread among sponges. In addition, new isolates obtained were closely related to D. spongiiphila. Compound Specific Isotope Analysis (CSIA) of 2,6-dibromophenol and its dehalogenation products revealed that carbon isotopic enrichment factors for sponge-associated bacterial dehalogenation are identical, probably due to a high similarity in the dehalogenating mechanism. The results support the hypothesis that dehalogenating microorganisms are widely distributed within marine sponges. The association between host sponge and associated microbiota is probably driven by the unique organohalide chemistry. At the same time the research of dehalogenating sponge-associated microorganism provided a unique setting for a deeper understanding into microbe-animal associations that could potentially be an ancient symbiosis
Subject
(authority = RUETD)
Topic
Environmental Sciences
Subject
(authority = ETD-LCSH)
Topic
Isotopes
Subject
(authority = ETD-LCSH)
Topic
Sponges
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_6834
PhysicalDescription
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (xv, 149 p. : ill.)
Note
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Ph.D.
Note
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Includes bibliographical references
Note
(type = statement of responsibility)
by Isabel Del Carmen Gray
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3WD42JT
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Gray
GivenName
Isabel
MiddleName
Del Carmen
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2015-10-02 11:07:39
AssociatedEntity
Name
Isabel Gray
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2015-10-31
DateTime
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(point = end)
2017-10-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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