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Purifications of small molecules that regulate neuronal cell-lineage specification

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TitleInfo
Title
Purifications of small molecules that regulate neuronal cell-lineage specification
Name (type = personal)
NamePart (type = family)
Hamod
NamePart (type = given)
Aula
NamePart (type = date)
1982-
DisplayForm
Aula Hamod
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Pang
NamePart (type = given)
Zhiping P
DisplayForm
Zhiping P Pang
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
There are certain important factors that are essential for specification, development and maintenance vertebrate nervous system. During development these factors regulate axonal growth, dendrite pruning, cell fate decision and different other neuronal functions. In adult nervous system, they modulate synaptic activity and regulate neuronal connectivity. Cultivation of mammalian cells for production of recombinant protein has wide scientific and clinical applications because it offers many advantages especially the posttranslational modification. Human embryonic kidney (HEK) 293 cell lines expressing gene of interests will be constructed using calcium phosphate transfection of expression vectors. I have constructed stable cell lines for expressing large quantity of stable neurotrophic and neurological fate determinant factors including: Brain derived neurotrophic factor (BDNF),Glial derived neurotrophic factor (GDNF), Neurotrophin 3(NT3), Noggin and Sonic the hedgehog (SHH).This recombinant proteins were successfully purified by affinity chromatography and are tested on the conversion of induced pluripotent stem (iPS) cells into specific type of neurons, we found that some of the purified proteins are capable of support neuronal differentiation. These neurons have complex morphology, mature synaptic marker such as synapsin, and exhibit mature membrane properties and synaptic activity. We conclude that mammalian cell produced recombinant factors can be used to regulate human neuronal maturation and cell type specification.
Subject (authority = RUETD)
Topic
Physiology and Integrative Biology
Subject (authority = ETD-LCSH)
Topic
Nervous system
Subject (authority = ETD-LCSH)
Topic
Neurons
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6643
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (vii, 67 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Aula Hamod
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T37P91CX
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Hamod
GivenName
Aula
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-08-04 02:48:47
AssociatedEntity
Name
AULA HAMOD
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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